{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T06:51:03Z","timestamp":1760079063217},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":7249,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1985,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of highly purified <jats:italic>Pseudomonas aeruginosa<\/jats:italic> cytotoxin were investigated on cultured pulmonary artery endothelial cells. This toxin dose\u2010dependently (7.5\u201360 \u03bcg\/ml) and time\u2010dependently (20\u201375 minutes) stimulated the release of radiolabeled arachidonic acid and metabolites and the synthesis of prostacyclin in the absence of overt cell damage (no enhanced lactate dehydrogenase [LDH] release). Preincubation of the toxin with neutralizing antibodies abolished the effect. The toxin response on endothelial cells required extracellular calcium but not magnesium and was accompanied by a calcium influx. Interference with intracellular calcium function by TMB 8 or with (calcium)\u2010calmodulin function by trifluoperazine and W7 dose\u2010dependently reduced the cytotoxin mediated synthesis of prostacyclin. Calcium channel blockers (nimodipine, diltiazem, verapamil, D 888), however, were ineffective in this system. Following addition of cytotoxin to endothelial cells, an increased passive permeability for small marker molecules (potassium, <jats:sup>45<\/jats:sup>calcium, <jats:sup>3<\/jats:sup>H\u2010sucrose), but not for large ones (<jats:sup>3<\/jats:sup>H\u2010inulin, <jats:sup>3<\/jats:sup>H\u2010dextran, LDH) was noted, suggesting that cytotoxin creates discrete hydrophilic transmembrane lesions of about 0.5\u20101.5 nm in diameter. These data are compatible with the notion that <jats:italic>Pseudomonas aeruginosa<\/jats:italic> cytotoxin triggers the arachidonic acid pathway in cultured pulmonary artery endothelial cells by calcium influx and suggest that this calcium influx may proceed through toxin created transmembrane lesions.<\/jats:p>","DOI":"10.1002\/jcp.1041230111","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T13:50:22Z","timestamp":1109425822000},"page":"64-72","source":"Crossref","is-referenced-by-count":53,"title":["<i>Pseudomonas aeruginosa<\/i> cytotoxin stimulates prostacyclin production in cultured pulmonary artery endothelial cells: Membrane attack and calcium influx"],"prefix":"10.1002","volume":"123","author":[{"given":"Norbert","family":"Suttorp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Seeger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Uhl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frieder","family":"Lutz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ladislaus","family":"Roka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(82)90040-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(79)90111-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.9.2514"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-171403-1.50006-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.9.5475"},{"key":"e_1_2_1_7_1","first-page":"672","article-title":"Inhibition of polymorphonuclear leukocyte adherence by prostacyclin","volume":"95","author":"Boxer L. 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