{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T21:21:59Z","timestamp":1772745719474,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":7096,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1985,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Both fluorescence microscopy and fluorometric analysis techniques have been used to characterize insulin receptor capping in IM\u20109 human lymphoblastoid cells. Morphologically, insulin caps appear similar to lectin or antiimmuno\u2010globulin\u2010induced caps displaying a preferential accumulation of actin, myosin, and actin\u2010binding protein directly underneath the cap structure. Using the fluorescent calcium indicator quin2 we have detected no change in the calcium activity following insulin stimulation. However, in the presence of a number of calmodulin inhibitors, such as W\u20105, W\u20107, W\u201012, and trifluoperazine (TFP), insulin capping is significantly inhibited, which implies that a calmodulin\u2010regulated process is involved. Using double immuonofluorescence microscopy, we have found that the calmodulin\u2010dependent myosin light chain kinase (MLCK) is concentrated directly beneath insulin caps. Upon treatment with trifluoperazine (TFP), the redistribution of both MLCK and insulin receptors are inhibited concomitantly. Our data indicate that the calmodulin\u2010dependent myosin light chain kinase may be directly responsible for the activation of actomyosin\u2010mediated contractility during insulin receptor capping.<\/jats:p>","DOI":"10.1002\/jcp.1041240308","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T12:52:18Z","timestamp":1109422338000},"page":"403-410","source":"Crossref","is-referenced-by-count":10,"title":["Insulin receptor capping and its correlation with calmodulin\u2010dependent myosin light chain kinase"],"prefix":"10.1002","volume":"124","author":[{"given":"Mary H.","family":"Majerick","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilly Y. 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