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The major [<jats:sup>32<\/jats:sup>PO<jats:sub>4<\/jats:sub>]\u2010labeled protein immunoprecipitated with antiphosphotyrosine antibodies from EGF\u2010stimulated A431 cells was the EGF receptor. Both the quantity of [<jats:sup>32<\/jats:sup>PO<jats:sub>4<\/jats:sub>]\u2010labeled proteins were reduced by the addition during EGF stimulation of trifluoperazine (TFP). TFP had little effect on the binding, internalization, and processing of [<jats:sup>125<\/jats:sup>I]\u2010EGF. In addition to the effects on phosphorylation, TFP inhibited cell growth both in the presence and absence of EGF. Morphologically, TFP blocked EGF\u2010induced ruffling. TFP did not alter the EGF\u2010 stimulated phosphatidylinositol turnover. In an in vitro experiment using A431 cell membranes, TFP did not inhibit phosphorylation of the EGF receptor.<\/jats:p>","DOI":"10.1002\/jcp.1041240321","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T12:52:18Z","timestamp":1109422338000},"page":"499-506","source":"Crossref","is-referenced-by-count":2,"title":["Inhibition of epidermal growth factor\u2010induced phosphorylation by trifluoperazine"],"prefix":"10.1002","volume":"124","author":[{"given":"Alonzo H.","family":"Ross","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Damsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul D.","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frances","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Vance","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"15162","DOI":"10.1016\/S0021-9258(18)33407-0","article-title":"Insulin\u2010stimulated tyrosine phosphorylation of the insulin receptor in detergent extracts of human placental membranes","volume":"257","author":"Avruch J.","year":"1982","journal-title":"J. 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