{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:36:09Z","timestamp":1790984169190,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":7066,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1985,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A method is described for quantitative measurement of lymphocyte transmembrane electrical potential difference (\u03a8) by flow cytometric recording of the oxonol dye fluorescence of single cells. Both the simultaneous collection and analysis of multiple optical parameters and the use of a negatively charged oxonol probe allowed more accurate measurement of \u03a8 than may be obtained by bulk cell suspension techniques employing cationic voltage indicators. Mouse spleen and human blood lymphocyte \u03a8 was calculated to be \u221270 mV. T and B lymphocytes maintain a constant \u03a8 as extracellular K<jats:sup>+<\/jats:sup> is varied from 2 to 10 mM and the deviation from K<jats:sup>+<\/jats:sup> equilibrium potentials (E<jats:sub>k<\/jats:sub>) is shown to result from Na<jats:sup>+<\/jats:sup> permeability. At [K<jats:sup>+<\/jats:sup>]<jats:sub>o<\/jats:sub> values greater than 10 mM, lymphocytes behave as K<jats:sup>+<\/jats:sup> electrodes. Examination of lymphocyte subsets showed that hyperpolarization induced by the Ca<jats:sup>2+<\/jats:sup> ionophore A23187 occurs only in T cells. This response was identified as activation of a Ca<jats:sup>2+<\/jats:sup>\u2010sensitive K<jats:sup>+<\/jats:sup> channel by pharmacologic manipulations. Hence, T cells depolarized by 4\u2010aminopyridine (4\u2010AP, 10 mM) were observed to return to resting \u03a8 by A23187\u2010induced elevation of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. Cells depolarized by quinine (100 \u03bcM) were unaffected by A23187. The Ca<jats:sup>2+<\/jats:sup>\u2010activated channel does not contribute to resting \u03a8 in T cells since it may be selectively blocked by quinine (20 \u03bcM) or modulated by calmodulin antagonists (5 \u03bcM trifluperazine) without affecting resting \u03a8.<\/jats:p>","DOI":"10.1002\/jcp.1041250110","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T12:57:58Z","timestamp":1109422678000},"page":"72-81","source":"Crossref","is-referenced-by-count":136,"title":["Lymphocyte membrane potential and Ca<sup>2+<\/sup>\u2010sensitive potassium channels described by oxonol dye fluorescence measurements"],"prefix":"10.1002","volume":"125","author":[{"given":"H. Alexander","family":"Wilson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Chused","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(82)90182-7"},{"key":"e_1_2_1_3_1","first-page":"1","article-title":"Determination of the proton electrochemical gradient across biological membranes","volume":"13","author":"Azzone G. F.","year":"1984","journal-title":"Cur. Top. Biophys. 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