{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T01:58:36Z","timestamp":1697939916838},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6578,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1987,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have hypothesized that two of the endogenously synthsized endometrial prostaglandins (PGs), prostaglandin F<jats:sub>2\u03b1<\/jats:sub> (PGF2\u03b1), and prostaglandin E<jats:sub>1<\/jats:sub> (PGE<jats:sub>1<\/jats:sub>), play a regulatory role in growth control of the rabbit endometrium. PGF<jats:sub>2\u03b1<\/jats:sub> increases DNA synthesis and PGE<jats:sub>1<\/jats:sub> inhibits that effect. Primary cultures of rabbit endometrial cells were used to examine the possible role of these PGs in the mechanism of action of 17\u03b2\u2010estradiol on DNA synthesis. Towards this end, binding, second messenger and DNA synthesis experiments were performed. 17\u03b2\u2010estradiol stimulation restulted in a time dependent (optimal: approximately 6 h) and 17\u03b2\u2010estradiol concentration dependent (optimal: approximately 10<jats:sup>\u22127<\/jats:sup> M 17\u03b2\u2010estradiol in phenol red\u2010containing medium) increase in [<jats:sup>3<\/jats:sup>H]PGF<jats:sub>2\u03b1<\/jats:sub> binding. Scatchard type analysis of the binding data revealed an increase in receptor number while the receptor affinity for [<jats:sup>3<\/jats:sup>H]PGF<jats:sub>2\u03b1<\/jats:sub> remained the same as in the control treated cultures. This 17\u03b2\u2010estradiol stimulated increase in PGF<jats:sub>2\u03b1<\/jats:sub> receptor allowed a suboptimal concentration of PGF<jats:sub>2\u03b1<\/jats:sub> (10<jats:sup>\u22129<\/jats:sup>M) to increase intracellular levels of inositol polyphosphates, while by itself this concentration of PGF<jats:sub>2\u03b1<\/jats:sub> caused no significant change in intracellular inositol polyphosphate levels. 17\u03b2\u2010estradiol, alone among the several studied steroid hormones, could increase [<jats:sup>3<\/jats:sup>H]PGF<jats:sub>2\u03b1<\/jats:sub> binding. Proliferation studies revealed that, in these primary cultures of rabbit endometrium, 17\u03b2\u2010estradiol could increase DNA synthesis but not in the presence of indomethacin, unless PGF<jats:sub>2\u03b1<\/jats:sub> was addd to the medium at a concentration (10<jats:sup>\u22129<\/jats:sup>M) near or above what is normally accumulated in the medium by these cultures. In the absence of 17\u03b2\u2010estradiol stimulation, addition of these same low concentrations of PGF<jats:sub>2\u03b1<\/jats:sub> had no effect on DNA synthesis. Apparently, through its effect on the PGF<jats:sub>2\u03b1<\/jats:sub> receptor, 17\u03b2\u2010estradiol enhances the PGF<jats:sub>2\u03b1<\/jats:sub> stimulated DNA synthesis response approximately 100 fold. The DNA synthesis inducd by 17\u03b2\u2010estradiol can be inhibited by PGE<jats:sub>1<\/jats:sub>, as can PGF<jats:sub>2\u03b1<\/jats:sub>\u2010induced DNA synthesis. We propose that 17\u03b2\u2010estradiol may be mediating its mitogenic effect through an alteration of the prostglandin agonist:antagonist control of proliferation in rabbit endometrial cultures. In additon we suggest that, if 17\u03b2\u2010estradiol acts to increase PGF<jats:sub>2\u03b1<\/jats:sub> receptors as part of its mode of action, this may be of importance in other tissues possessing both prostaglandin and 17\u03b2\u2010estradiol receptos.<\/jats:p>","DOI":"10.1002\/jcp.1041300216","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T12:54:36Z","timestamp":1109422476000},"page":"292-300","source":"Crossref","is-referenced-by-count":13,"title":["Requirement of prostaglandin F<sub>2\u03b1<\/sub> in 17\u03b2\u2010estradiol stimulation of DNA synthesis in rabbit endometrial cultures"],"prefix":"10.1002","volume":"130","author":[{"given":"D. J.","family":"Orlicky","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Lieberman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. E.","family":"Gerschenson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(74)90014-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-1-138"},{"key":"e_1_2_1_4_1","first-page":"132","article-title":"Inhibitory effect of phorbol 12\u2010myristate 13\u2010acetate (PMA) and 1\u2010oleoyl\u20102\u2010acetylglycerol (OAG) on the decidual cell reaction (DCR) in rats","volume":"34","author":"Feyles V.","year":"1986","journal-title":"Biol. Reprod."},{"key":"e_1_2_1_5_1","first-page":"2873","article-title":"Diethylistilbesterol and progesterone regulation of cultured rabbit endometrial cell growth","volume":"34","author":"Gerschenson L. E.","year":"1974","journal-title":"Cancer Res."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(79)90002-X"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod18.1.148"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)91847-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod23.5.955"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod29.5.1069"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod27.1.253"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/3.6.703"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(73)90115-9"},{"key":"e_1_2_1_14_1","first-page":"103","volume-title":"Estrogens in the Environment","author":"Martin L.","year":"1980"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00216228"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90515-1"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-011-7855-6_10"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(83)90147-8"},{"key":"e_1_2_1_19_1","unstructured":"Orlicky D. J.(1985)Involvement of prostaglandins F2\u03b1and E1with rabbit endometrium. Ph. D. Thesis University of Colorado."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041270108"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041270109"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041280116"},{"key":"e_1_2_1_23_1","unstructured":"Orlicky D. J. Lieberman R. Williams C. andGerschenson L. E.(1986d)Effect of phorbol ester on prostaglandin regulation of proliferation in rabbit endometrial cells. Submitted for publication."},{"key":"e_1_2_1_24_1","first-page":"1391","article-title":"Prostaglandins and implantation in the rat","volume":"8","author":"Phillips C. A.","year":"1980","journal-title":"Adv. Prostaglandin Thromboxane Res."},{"key":"e_1_2_1_25_1","first-page":"53","volume-title":"The Physiology and Biochemistry of the Uterus in Pregnancy and Labor","author":"Riemer R. K.","year":"1986"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910360218"},{"key":"e_1_2_1_27_1","first-page":"801","article-title":"Relationship between estrogen, prostgalndin F2\u03b1 and histamine in delayed implantation in the mouse","volume":"81","author":"Saksena S. K.","year":"1976","journal-title":"Acta Endocrinol."},{"key":"e_1_2_1_28_1","first-page":"153","article-title":"Prostaglandin(s) as inductive factor of decidualization in the rat uterus","volume":"6","author":"Sananes N.","year":"1976","journal-title":"Mol. Cell. Endocrinol."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1949.tb27297.x"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.8.3786"},{"key":"e_1_2_1_31_1","first-page":"169","volume-title":"Estrogens in the Environment","author":"Sonnenschein C.","year":"1980"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(85)90265-1"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod29.3.671"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(83)90269-2"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0470391"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-3-1011"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-4-1537"},{"key":"e_1_2_1_38_1","first-page":"312","volume-title":"Handbook of Biochemistry and Molecular Biology","author":"Willis A. L.","year":"1976"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041300216","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041300216","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T00:21:31Z","timestamp":1697847691000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041300216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,2]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1987,2]]}},"alternative-id":["10.1002\/jcp.1041300216"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041300216","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,2]]}}}