{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T20:16:20Z","timestamp":1758399380473},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6550,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1987,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The characterization and purification of the growth\u2010promoting activity present in rat submaxillary gland extracts, known to be required for the proliferation of adipose precursor cells in serum\u2010free medium, have been undertaken. Fractionation of the extracts by ion\u2010exchange chromatography, gel filtration, and affinity chromatography on immobilized benzamidine allowed the copurification of the mitogenic activity with two distinct arginylesteropeptidases of apparent molecular weight 25,000; one of these enzymes has been purified to homogeneity and shown to be immunologically related to tonin, a well\u2010characterized kallikreinlike protease from submaxillary gland. The specificity of both enzymes was similar to that of plasma and glandular kallikreins, as indicated by the relative rates of hydrolysis of peptide p\u2010nitroanilide substrates. Prior treatment of the kallikreinlike proteases with phenylmethylsulfonylfluoride or aprotinin abolished completely both mitogenic and arginylesteropeptidase activities, indicating that enzymatic activity was essential for the manifestation of their growth\u2010promoting ability. The kallikreinlike proteases from rat submaxillary gland were able to replace thrombin to support the proliferation of Chinese hamster lung fibroblasts in serum\u2010free medium. These results underline the role of proteases in controlling cell growth and are discussed in light of adipose tissue development.<\/jats:p>","DOI":"10.1002\/jcp.1041300307","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T11:43:22Z","timestamp":1109418202000},"page":"352-360","source":"Crossref","is-referenced-by-count":17,"title":["Growth\u2010promoting activity in serum\u2010free medium of kallikreinlike arginylesteropeptidases from rat submaxillary gland"],"prefix":"10.1002","volume":"130","author":[{"given":"R. M.","family":"Catalioto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"N\u00e9grel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Gaillard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Ailhaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00230992"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2380115"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91165-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.150.3701.1307"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.156.3779.1253"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/28.8.7003006"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"7287","DOI":"10.1016\/S0021-9258(18)50317-3","article-title":"The relationship between glandular kallikrein and growth factor\u2010processing proteases of mouse submaxillary gland","volume":"254","author":"Bothwell M. A.","year":"1979","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1976.tb07438.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1983.tb07614.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/227170a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90189-0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6480"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(81)90107-5"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90412-5"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(82)90368-8"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2380123"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1978.235.3.E279"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041230212"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02626647"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(76)90260-9"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(71)90092-9"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1002\/j.1460-2075.1982.tb01231.x","article-title":"Differentiation of Ob17 preadipocytes to adipocytes: Requirement of adipose conversion factor(s) for fat cell cluster formation","volume":"6","author":"Grimalidi P.","year":"1982","journal-title":"Embo J."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041110202"},{"key":"e_1_2_1_25_1","first-page":"607","article-title":"Adipose tissue cellularity and human obesity","volume":"26","author":"Hirsch J.","year":"1977","journal-title":"Clin. Endocrinol. Metab."},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.3.1.81"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00356a002"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(84)90074-4"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/307555a0"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.1.183"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107115"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/298476a0"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041070205"},{"key":"e_1_2_1_36_1","first-page":"113","volume-title":"Chemistry and Biology of Thrombin","author":"McKay D. B.","year":"1977"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6162199"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(81)90046-1"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.12.6054"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041090303"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(71)90108-4"},{"key":"e_1_2_1_42_1","first-page":"490","article-title":"Proteases act synergistically with low molecular weight inducers to stimulate mouse erythroleukemia cell differentiation","volume":"11","author":"Scher W.","year":"1983","journal-title":"Exp. Hematol."},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/227843a0"},{"key":"e_1_2_1_44_1","first-page":"523","volume-title":"Cold Spring Harbor Conferences on Cell Proliferation","author":"Serrero G.","year":"1979"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(81)90103-0"},{"key":"e_1_2_1_46_1","doi-asserted-by":"crossref","first-page":"9147","DOI":"10.1016\/S0021-9258(19)52521-2","article-title":"Electrophoretic heterogeneity and polypeptide chain structure of the \u03b3\u2010subunit of mouse submaxillary 7S nerve growth factor","volume":"256","author":"Thomas K. A.","year":"1981","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_47_1","first-page":"41","article-title":"Adipose conversion of Ob17 cells and hormone\u2010related events","volume":"1","author":"Vannier C.","year":"1985","journal-title":"Int. J. Obes., 9, suppl."},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(83)90219-7"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(85)90039-4"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041300307","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041300307","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041300307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T11:56:31Z","timestamp":1697889391000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041300307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,3]]},"references-count":48,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1987,3]]}},"alternative-id":["10.1002\/jcp.1041300307"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041300307","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,3]]}}}