{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:50:58Z","timestamp":1758279058205},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6519,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1987,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The role of magnesium ions in the differentiation of human promyelocytic leukemia HL\u201060 cells was investigated. When HL\u201060 extracellular magnesium was deficient (less than 0.01 mM), the total intracellular magnesium content and [<jats:sup>3<\/jats:sup>H] leucine incorporation rates decreased to 61 and 28%, respectively, on day 3. When the cells were treated with various inducers (100 nM 1\u03b1, 25 dihydroxyitamine D<jats:sub>3<\/jats:sub> (1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>), 100 nM \u03b2\u2010all\u2010trans retinoic acid (RA), 20 nM 12\u2010o\u2010tetradecanoyl phorbol\u201013\u2010acetate (TPA), 1.25% dimethylsulfoxide (DMSO) and 30 nM aclacinomycin (AcM)) in magnesium\u2010deficient medium, the expression of differentiation\u2010related phenotypes (nitroblue tetrazolium (NBT) reducing ability, nonspecific esterase (NSE) activity and monoclonal antibody, OKM1 binding activity) was almost completely inhibited. After a 2\u2010day treatment with 100 nM 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> in magnesium\u2010deficient medium, the expression of differentiation\u2010related phenotypes was restored by further incubation in the absence of inducer in standard magnesium medium (0.4 mM). These results suggested that magnesium deprivation inhibited the expression of HL\u201060 differentiation\u2010related phenotypes but not their commitment to differentiation. These phenotypes were expressed without inducer in standard magnesium medium after a 2\u2010day simultaneous treatment with 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> and cyclohexamide (protein synthesis inhibitor) in magnesium\u2010deficient medium, but not after simultaneous pretreatment with 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> and \u03b1 \u2010amanitin (RNA synthesis inhibitor). Thus, it was suggested that the magnesium\u2010requiring step in HL\u201060 cell differentiation is in protein but not mRNA synthesis. This conclusion is supported by the findings that changes in c\u2010myc and c\u2010fms mRNA levels in HL\u201060 cells treated with 100 nM 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> in magnesium\u2010deficient medium and those in standard magnesium medium were the same. In addition, dibutyryl cyclic adenosine monophosphate (dbc AMP) could restore expression of differentiation\u2010related phenotypes inhibited by magnesium deprivation but not those inhibited by cyclohexamide, even though magnesium deprivation inhibited protein synthesis as much as did cyclohexamide. This suggests that magnesium\u2010requiring step in HL\u201060 cell differentiation is different from that inhibited by cyclohexamide.<\/jats:p>","DOI":"10.1002\/jcp.1041310109","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T11:43:39Z","timestamp":1109418219000},"page":"50-57","source":"Crossref","is-referenced-by-count":9,"title":["Magnesium deprivation inhibits the expression of differentiation\u2010related phenotypes in human promyelocytic leukemia HL\u201060 cells"],"prefix":"10.1002","volume":"131","author":[{"given":"Toshiro","family":"Okazaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Mochizuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaro","family":"Tashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyoshi","family":"Sawada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruto","family":"Uchino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040820112"},{"issue":"1","key":"e_1_2_1_3_1","first-page":"200a","article-title":"The c\u2010fms proto\u2010oncogene in normal bone marrow and deletion in the 5q syndrome","volume":"64","author":"Bunn H. 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