{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:34:00Z","timestamp":1777451640267,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6519,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1987,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the effects of reduced temperature, the pH elevators NH<jats:sub>4<\/jats:sub> CI, monensin, and HEPES (N\u20102\u2010hydroxy\u2010ethylpiperazine\u2010N'\u20102\u2010ethanesulfonic acid) buffer, as well as the metabolic poisons NaF\/KCN on transport of the fluid phase pinocytic marker, horseradish peroxidase (HRP), to lysosomes in Chinese hamster ovary (CHO) cells. In cell fractionation experiments, these agents appeared to block HRP transit at specific point(s) from \u201cearly\u201d to \u201clate\u201d (i.e., low to high density) prelysosomal vesicles and lysosomes. Reduced temperature (17\u00b0C) most strongly inhibited HRP transport from low density, early endosomes to lysosomes. In long\u2010term HRP uptakes at 17\u00b0C, marked peroxidase accumulation occurred both in early endosomes and in lysosomes. Loss (reversible pinocytosis) of HRP from \u201cvery early\u201d endosomes occurred at 17\u00b0C. All three pH elevators including the common media supplement HEPES buffer inhibited transit of internalized HRP into lysosomes. For all three pH elevators, inhibition was most pronounced at the \u201cearly\u201d endosome stage. The respiratory inhibitors NaF\/KCN also inhibited transport most strongly at the early endosome stage. Together these results suggest that \u201cearly\u201d steps in the endocytic transport of HRP are the most sensitive and that the conditions tested may exert direct effects on the processing of endocytic vesicles.<\/jats:p>","DOI":"10.1002\/jcp.1041310110","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T11:43:39Z","timestamp":1109418219000},"page":"58-63","source":"Crossref","is-referenced-by-count":34,"title":["Effects of temperature, pH elevators, and energy production inhibitors on horseradish peroxidase transport through endocytic vesicles"],"prefix":"10.1002","volume":"131","author":[{"given":"Peter C.","family":"Sullivan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea L.","family":"Ferris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Storrie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.93.3.632"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.93.3.523"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1242\/jcs.83.1.119","article-title":"Characterization of early compartments in fluid phase pinocytosis: A cell fractionation study","volume":"83","author":"Casey K. 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