{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:42:44Z","timestamp":1758278564016},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6397,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1987,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chinese hamster embryo fibroblast cells (CHEF\/18) possess a plasma membrane\u2010associated, amiloride\u2010sensitive Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> antiporter that affects intracellular pH (pH<jats:sub>i<\/jats:sub>) and is activated by growth factor addition. Our results using <jats:sup>14<\/jats:sup>C\u2010benzoic acid distribution indicate that both epidermal growth factor (EGF) and thrombin are capable of causing rapid rises in the pH<jats:sub>i<\/jats:sub> of CHEF\/18 cells. The maximal shift induced by these factors is 0.20 to 0.25 pH units above the basal unstimulated level. Distinctive differences were observed between the modes of action of these two growth factors. Sequential additions revealed that the rise in pH<jats:sub>i<\/jats:sub> due to EGF was additive with that caused by diacylglycerols (DAG), while that of thrombin was not. Furthermore, exposure of cells to the phorbol ester PMA for a prolonged period of time in order to down\u2010regulate protein kinase C (pkC), or treatment with the pkC inhibitor H\u20107, abolished the pH<jats:sub>i<\/jats:sub> response to thrombin but not to EGF. In contrast, incubation of cells in nominally calcium\u2010free medium or with the calmodulin antagonists W\u20107 or trifluoperazine (TFP) decreased only the ability of EGF to cause changes in pH<jats:sub>i<\/jats:sub>. These data suggest that there are two distinct mechanisms for activation of the Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> antiporter in CHEF\/18 fibroblast cells and thus provide an example of the use of alternative modes for the modulation of intracellular processes.<\/jats:p>","DOI":"10.1002\/jcp.1041320216","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T12:09:22Z","timestamp":1109419762000},"page":"311-317","source":"Crossref","is-referenced-by-count":38,"title":["Both protein kinase C and calcium mediate activation of the Na<sup>+<\/sup>\/H<sup>+<\/sup> antiporter in Chinese hamster embryo fibroblasts"],"prefix":"10.1002","volume":"132","author":[{"given":"Sharon S.","family":"Ober","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur B.","family":"Pardee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3090687"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.242.3.C131"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2200345"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1984.246.4.R409"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240260306"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/326800a0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.8.3937"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041050105"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90029-7"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.3.887"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1016\/S0021-9258(18)89586-2","article-title":"Amiloride directly inhibits growth factor tyrosine kinase activity","volume":"260","author":"Davis R. J.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.83.3.341"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869680"},{"key":"e_1_2_1_15_1","first-page":"571","article-title":"Activity\u2010structure relationship of calmodulin antagonists: Naphthalenesulfonamide derivatives","volume":"20","author":"Hidaka H.","year":"1981","journal-title":"Molec. Pharm."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.7.4354"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"14321","DOI":"10.1016\/S0021-9258(17)42594-4","article-title":"Serotonin secretion from human platelets may be modified by Ca+2\u2010activated phospholipid\u2010dependent myosin phosphorylation","volume":"259","author":"Inagaki M.","year":"1984","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89453-4"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S0021-9258(20)71168-3","article-title":"A bicarbonate\u2010dependent process inhibitable by disulfonic stilbenes and a Na+\/H+ exchange mediate 22\/Na+ uptake into cultured bovine corneal endothelium","volume":"260","author":"Jentsch T. J.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(84)80362-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"5809","DOI":"10.1016\/S0021-9258(18)91086-0","article-title":"Growth factor action and intracellular pH regulation in fibroblasts","volume":"259","author":"L'Allemain G.","year":"1984","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"4877","DOI":"10.1016\/S0021-9258(18)89153-0","article-title":"Role of a Na+\u2010dependent Cl\/HCO3\n                  \u2212 exchange in regulation of intracellular pH in fibroblasts","volume":"260","author":"L'Allemain G.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6435"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/322552a0"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3014651"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.11.3537"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041250330"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(84)80213-2"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3018928"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01542849"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)81527-7"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90466-8"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041320216","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041320216","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T04:17:29Z","timestamp":1697861849000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041320216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,8]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1987,8]]}},"alternative-id":["10.1002\/jcp.1041320216"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041320216","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,8]]}}}