{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T18:45:43Z","timestamp":1771872343956,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6397,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1987,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In human umbilical vein and bovine aortic endothelial cells in culture c\u2010<jats:italic>sis<\/jats:italic> gene expression and secretion of platelet\u2010derived growth factor (PDGF) has been previously demonstrated. We now report the presence of PDGF\u20101 and PDGF\u20102\/<jats:italic>sis<\/jats:italic> mRNA transcripts in primary cultures of human iliac artery endothelial cells (HIA\u2010EC). Concomitantly, these cells synthesize and secrete PDGF\u2010like proteins identified by direct immunoprecipitation with specific PDGF antiserum. The PDGF proteins secreted by HIA\u2010EC have molecular weights of 31 and 35 kd under nonreducing conditions. Upon reduction these proteins are converted to the monomeric 15\u2010 and 16\u2010kd forms. Conditioned media derived from HIA\u2010EC stimulated the incorporation of <jats:sup>3<\/jats:sup>H\u2010thymidine by 3T3 cells and competed with <jats:sup>125<\/jats:sup>I\u2010PDGF for its binding to 3T3 cell membrane receptors. The biologic activity was stable to heating at 100\u00b0C for 10 min and sensitive to reducing agents, properties similar to those of authentic PDGF. Production of PDGF\u2010like mitogen by the human arterial endothelial cells may play an important role in the paracrine modulation of arterial wall regeneration following vascular injury.<\/jats:p>","DOI":"10.1002\/jcp.1041320228","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T12:09:22Z","timestamp":1109419762000},"page":"376-380","source":"Crossref","is-referenced-by-count":25,"title":["Human iliac artery endothelial cells express both genes encoding the chains of platelet\u2010derived growth factor (PDGF) and synthesize PDGF\u2010like mitogen"],"prefix":"10.1002","volume":"132","author":[{"given":"N. M.","family":"Sitaras","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Sariban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Pantazis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Zetter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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