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Response to various additives has been analyzed for growth\u2010promoting effect when added to a basal medium containing insulin, cholera toxin, and BSA. A consistent observation has been the effect of EGF and cortisol in growth stimulation of human breast epithelial cells, while separately, each additive elicited only a small response. Under this condition, employing EGF and cortisol combinations, these cells gave rise to organized colonies consisting of clusters of cells, usually spherical, without any duct\u2010like extensions. Ultrastructural and immunocytochemical studies, using a panel of monoclonal and polyclonal antibodies, have shown that cell types and features that can be identified in the original breast tissue can also be delineated in the progeny populations. The topographical feature, consisting of lumina surrounded by a single inner layer of epithelial cells and an outer layer of basal\/myoepithelial cells, can be re\u2010created in the collagen gel system starting from small clumps of cells.<\/jats:p>","DOI":"10.1002\/jcp.1041330205","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T12:21:53Z","timestamp":1109420513000},"page":"228-234","source":"Crossref","is-referenced-by-count":26,"title":["Human breast epithelial cells in serum\u2010free collagen gel primary culture: Growth, morphological, and immunocytochemical analysis"],"prefix":"10.1002","volume":"133","author":[{"given":"Jason","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun","family":"Balakrishnan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Hamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel J.","family":"Elias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Rosenau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig W.","family":"Beattie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tapas K.","family":"Das Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sefton R.","family":"Wellings","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satyabrata","family":"Nandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"177","article-title":"Freeze\u2010substitution: A method applicable to routine surgical biopsies","volume":"80","author":"Ahlquist J.","year":"1972","journal-title":"Acta Pathol. 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