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The number of serotonin receptor types on the platelet membrane and the function of these receptors has not been conclusively demonstrated. The presence of different receptor types that may be altered or lost in disease or upon aging (in vitro storage or in vivo) could have significant physiological effects on platelet function. This report demonstrates that at least two receptor types are present on freshly prepared human platelets. However, after 3 to 4 days of storage in autologous plasma, the low\u2010affinity, high\u2010capacity serotonin receptor appears to be lost. This phenomenon probably accounts for some of the discrepancies reported in the literature. The high\u2010affinity receptor present in both freshly isolated and stored platelets binds about 9 \u00d7 10<jats:sup>3<\/jats:sup> serotonin molecules per platelet. Binding can be completely blocked by imipramine; however, some passive diffusion appears to occur even at the low level of extracellular serotonin concentrations employed in these studies (nanomolar range). The influx of serotonin into platelets appears to be poorly reversible, even in reserpine\u2010treated cells, where the extravesicular cytoplasmic concentration would be high. The loss of the low\u2010affinity serotonin receptor type reported in these studies may be directly or indirectly associated with the reduced responsiveness observed in stored platelets.<\/jats:p>","DOI":"10.1002\/jcp.1041370318","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T11:06:33Z","timestamp":1109415993000},"page":"521-528","source":"Crossref","is-referenced-by-count":4,"title":["Loss of low\u2010affinity serotonin receptors upon storage of human platelets"],"prefix":"10.1002","volume":"137","author":[{"given":"Gerald","family":"Soslau","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy","family":"Gillen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet","family":"Parker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(86)90073-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1969.sp008763"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/218137a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1969.sp008762"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.1973.tb05442.x"},{"key":"e_1_2_1_7_1","first-page":"342","article-title":"Buoyant density of platelets stored at room temperature as platelet concentrates","volume":"98","author":"Bolin R. 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