{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:08:23Z","timestamp":1756994903664},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5819,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1989,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rat submandibular cells treated with methylcholanthrene are able to be propagated in continuous culture while retaining \u03b2\u2010adrenergic responsiveness. A specific clone, RSMT\u2010A5, has been isolated and studied in detail. RSMT\u2010A5 cells possess \u03b2\u2010adrnergic receptors (BARS) as judged by [<jats:sup>3<\/jats:sup>H]\u2010dihydroalprenolol ([<jats:sup>3<\/jats:sup>H]\u2010DHA) binding studies. [<jats:sup>3<\/jats:sup>H]\u2010DHA binds to RSMT\u2010A5 membranes in a specific and saturable manner with respect to time and [<jats:sup>3<\/jats:sup>H]\u2010DHA concentration. Specific binding is saturable within three min of incubation, and a Scatchard analysis reveals a single class of high affinity binding sites with an equilibrium dissociation constant of 0.62 \u00b1 0.03 nM and a receptor density of 101 \u00b1 4 fmole\/mg protein. Antagonist competition studies indicate that the BARs are primarily of the \u03b2<jats:sub>2<\/jats:sub>\u2010subtype. The BARs are functional since isoproterenol stimulation results in an increased intracellular cAMP content, marked morphological change, and decreased cell volume and chloride content. These same responses can be evoked by treating RSMT\u2010A5 cells with 8\u2010bromo\u2010cAMP. Ion transport inhibitors such as bumetanide (an inhibitor of Na\/K\/C1 cotransport), SITS and DIDS (inhibitors of chloride\u2010bicarbonate exchange), amiloride (an inhibitor of Na\u2010H exchange), ouabain (an inhibitor of Na\/K\u2010ATPase), and dipyridamole and 9\u2010anthracene carboxylic acid (chloride channel blockers) fail to inhibit the isoproterenol\u2010stimulated change in chloride content. The effects of either isopro\u2010terenol or 8\u2010bromo\u2010cAMP on both chloride content and cell volume can be inhibited by the chloride channel blocker N\u2010phenylanthranilic acid, however. Taken together, our results indicate that RSMT\u2010A5 cells possess a \u03b2\u2010adrenergic receptor system which controls intracellular volume and chloride content by modulating transport processes that are 1) cAMP\u2010responsive and 2) inhibitable by the putative chloride channel blocker N\u2010phenylanthranilic acid.<\/jats:p>","DOI":"10.1002\/jcp.1041380312","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T11:11:33Z","timestamp":1109416293000},"page":"527-535","source":"Crossref","is-referenced-by-count":18,"title":["\u03b2\u2010adrenergic control of cell volume and chloride transport in an established rat submandibular cell line"],"prefix":"10.1002","volume":"138","author":[{"given":"Xinjun","family":"He","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Ship","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozai","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley M.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert B.","family":"Wellner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345870660S204"},{"key":"e_1_2_1_3_1","first-page":"2779","article-title":"In vitro transformation of submandibular gland epithelial cells and fibroblasts of adult rats by methylcholanthrene","volume":"33","author":"Brown A. 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