{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T15:49:39Z","timestamp":1770565779616,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5727,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Interleukin\u20104 (IL\u20104), which was originally identified as a B\u2010cell growth factor, has been shown to produce diverse effects on hemopoietic progenitors. The present study investigated the effects of purified recombinant murine IL\u20104 on early hemopoetic progenitors in methylcellulose culture. IL\u20104 supported the formation of blast cell colonies and small granulocyte\/macrophage (GM) colonies in cultures of marrow and spleen cells of normal mice as well as spleen cells of mice treated with 150 mg\/kg 5\u2010fluorouracil (5\u2010FU) 4 days earlier. When the blast cell colonies were individually picked and replated in cultures containing WEHI\u20103 conditioned medium and erythropoietin (Ep), a variety of colonies were seen, including mixed erythroid colonies, indicating the multipotent nature of the blast cell colonies supported by IL\u20104. To test whether or not IL\u20104 affects multipotent progenitors directly, we replated pooled blast cells in cultures under varying conditions. In the presence of Ep, both IL\u20103 and IL\u20104 supported a similar number of granulocyte\/erythrocyte\/macrophage\/megakaryocyte (GEMM) colonies. However, the number of GM colonies supported by IL\u20104 was significantly smaller than that supported by IL\u20103. When colony\u2010supporting abilities of IL\u20104 and IL\u20103 were compared using day\u20104 post\u20105\u2010FU spleen and day\u20102 post\u20105\u2010FU marrow cells, IL\u20104 supported the formation of fewer blast cell colonies than did IL\u20103. IL\u20104 and IL\u20106 revealed synergy in support of colony formation from day 2 post\u20105\u2010FU marrow cells. These results indicate that murine IL\u20104 is another direct\u2010acting multilineage colonystimulating factor (multi\u2010CSF), similar to IL\u20103, that acts on primitive hemopoietic progenitors.<\/jats:p>","DOI":"10.1002\/jcp.1041390303","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T10:20:32Z","timestamp":1109413232000},"page":"463-468","source":"Crossref","is-referenced-by-count":26,"title":["Murine B\u2010cell stimulatory factor\u20101 (BSF\u20101)\/Interleukin\u20104 (IL\u20104) is a multilineage colony\u2010stimulating factor that acts directly on primitive hemopoietic progenitors"],"prefix":"10.1002","volume":"139","author":[{"given":"Kenji","family":"Kishi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James N.","family":"Ihle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Urdal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makio","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"3852","DOI":"10.4049\/jimmunol.141.11.3852","article-title":"Synergistic effects of purified recombinant human and murine B cell growth factor\u20101\/IL\u20104 on colony formation in vitro by hematopoietic progenitor cells","volume":"141","author":"Broxmeyer H. 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