{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:33:14Z","timestamp":1790364794698,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5727,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We report here that epidermal growth factor (EGF) isolated from male mouse salivary glands is active as a potent chemoattractant for a rat heart vascular endothelial cell line (RHEC). Human EGF and transforming growth factor\u2010alpha (TGF\u2010alpha) produced in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    are also active as chemoattractants for these cells. The chemotactic response of the cells is induced by concentrations of murine EGF that ranged from 0.01 to 1.0 nM with similar specific activities for both the human EGF and TGF\u2010alpha. Other growth factors such as fibroblast growth factor (FGF), TGF\u2010beta, platelet\u2010derived growth factor (PDGF), insulin, or IGF were not active as chemoattractants for these cells. The chemotactic response exhibited similar kinetics to those reported for connective tissue cells responding to PDGF (2\u20136 h) and required both RNA and protein synthesis. However, in contrast to NIH\/3T3 cells responding to PDGF, the proliferating cultures of RHEC respond as well as growth\u2010arrested cultures in the chemotaxis assay. In in vivo studies of connective tissue regeneration it appears that EGF\u2010like factors are present in wound fluid collected from rats. Acid extracts of wound fluid contained a RHEC chemotactic activity that was neutralized with anti\u2010EGF antisera. These studies indicate that EGF\u2010like factors may function in vivo to control in part the angiogenic events that occur during tissue repair.\n                  <\/jats:p>","DOI":"10.1002\/jcp.1041390323","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T05:20:32Z","timestamp":1109395232000},"page":"617-623","source":"Crossref","is-referenced-by-count":113,"title":["EGF and TGF\u2010alpha are potent chemoattractants for endothelial cells and EGF\u2010like peptides are present at sites of tissue regeneration"],"prefix":"10.1002","volume":"139","author":[{"given":"Gary R.","family":"Grotendorst","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinao","family":"Soma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Takehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Charette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"467","article-title":"How is blood vessel growth regulated in normal and neoplastic tissue?","volume":"46","author":"Folkman J.","year":"1986","journal-title":"Cancer Res."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2432664"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/52.2.413"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1126\/science.472760","article-title":"Angiogenesis in the mouse cornea","volume":"205","author":"Muthukkaruppan V. R.","year":"1976","journal-title":"Science"},{"key":"e_1_2_1_6_2","first-page":"110","article-title":"Tumor angiogenesis activity in cells grown in tissue culture","volume":"36","author":"Klagsbrun M.","year":"1976","journal-title":"Cancer Res."},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/285041a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240290402"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6507"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6409"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00340a001"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2422759"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3669"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.92.2.584"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041130213"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.96.2.382"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.6.2330"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90221-6"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"7155","DOI":"10.1016\/S0021-9258(18)32345-7","article-title":"Transforming growth factor beta in human platelets: Identification of a major storage site, purification and characterization","volume":"258","author":"Assoian R. K.","year":"1983","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_20_2","first-page":"144","volume-title":"Methods of Enzymology, Vol. 147, Peptide Growth Factors","author":"Grotendorst G. R.","year":"1987"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90294-7"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1949.tb27297.x"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112243"},{"key":"e_1_2_1_24_2","first-page":"119","article-title":"Doxorubicin\u2010induced impairment of wound healing in rats","volume":"76","author":"Lawrence W. T.","year":"1986","journal-title":"JNCI"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.8.4001"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.12.4167"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109257"},{"key":"e_1_2_1_28_2","first-page":"203","volume-title":"Current Topics in Cellular Regulation","author":"Schiffmann E.","year":"1979"},{"key":"e_1_2_1_29_2","first-page":"702","article-title":"Wound healing: A comparative study of the histochemical changes in granulation tissue contained stainless steel wire mesh and polyvinyl sponge cylinders","volume":"46","author":"Schilling J. A.","year":"1959","journal-title":"Surgery"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9610(67)90388-1"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6572416"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-198602000-00006"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041230203"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.24.7773"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/319158a0"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90033-9"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110964"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90614-X"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.17.6020"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111810"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041390323","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041390323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T03:43:16Z","timestamp":1697946196000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041390323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,6]]}},"alternative-id":["10.1002\/jcp.1041390323"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041390323","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,6]]}}}