{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T13:41:49Z","timestamp":1698068509517},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5513,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A nonmalignant near\u2010diploid cell line m5s\/1M, established by Sasaki and Kodama (J. Cell. Res., 131:114\u2013122, 1987), was shown to respond to the epidermal growth factor (EGF). The m5s\/1M cells showed high sensitivity to post\u2010confluence inhibition of cell division and formed a uniform monolayer after the cells had become confluent. The addition of EGF resulted in loss of contact\u2010dependent inhibition of growth and caused a massive piling up of a multilayered array of cells after they had become confluent. When EGF was removed from the medium, the cell number decreased rapidly, and the cells formed a uniform monolayer at the density observed in the absence of EGF. m5S\/1M cells have high\u2010 and low\u2010affinity receptors for EGF (approximately 40,000 receptors per cell), and the apparent dissociation constants of the EGF\u2010binding reactions were 3.3 nM and 0.15 nM, respectively. The effect of EGF on the intracellular mobilization of Ca<jats:sup>2+<\/jats:sup> and the formation of inositol phosphates was studied by using the calcium\u2010sensitive fluorescent indicator fura 2 and [<jats:sup>3<\/jats:sup>H]inositol. EGF had no effect either on the mobilization of cytosolic free calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) or on the formation of inositol phosphates in m5\/1M cells, whereas bradykinin induced a rapid increase in both [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and inositol phosphates. Analysis of the gly\u2010cosphingolipid (GSL) composition of m5S\/1M cells showed that globotriaosyl\u2010ceramide (Gb<jats:sub>3<\/jats:sub>Cer), which is known to be a Burkitt lymphoma\u2010associated antigen, is specifically expressed in the EGF\u2010treated cells. The expression of Gb<jats:sub>3<\/jats:sub>Cer is dependent on the presence of EGF, with a reversible shift in GSL composition being observed in the presence or absence of EGF.<\/jats:p>","DOI":"10.1002\/jcp.1041420113","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T09:44:06Z","timestamp":1109411046000},"page":"99-107","source":"Crossref","is-referenced-by-count":0,"title":["Mitogenic response of near\u2010diploid mouse cell line m5S\/1M induced by epidermal growth factor"],"prefix":"10.1002","volume":"142","author":[{"given":"Sachihiko","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Umeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Nomura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuyuki","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohoru","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Arita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Utsumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao S.","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keizo","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90373-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(76)90054-0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2220195"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.004313"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0021-9258(19)41979-0","article-title":"Glycosphin golipids of human KB cells grown in monolayer, suspension, and synchronized cultures","volume":"250","author":"Chatterjee S.","year":"1975","journal-title":"J. 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