{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T22:47:49Z","timestamp":1698274069893},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5423,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1990,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The growth of the human leukemia cell line AML\u2010193 in a serum\u2010free medium is strictly dependent on the presence of the cytokine granulocyte\u2010macrophage colony\u2010stimulating factor (GM\u2010CSF), which is one of the major regulators of the myelomonocytic lineage. At present, little is known about the mechanisms by which this growth factor transduces the signal intracellularly. The results of this study demonstrate that GM\u2010CSF needs the operation of a Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchanger, which is located in the plasma membrane of almost every vertebrate cell. In fact, the GM\u2010CSF\u2010dependent proliferation of AML\u2010193 cells is strongly reduced in the presence of the amiloride analog EIPA, a specific inhibitor of the Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchanger. When acidified, AML\u2010193 cells are able to recover the original pH, in a Na<jats:sup>+<\/jats:sup>\u2010dependent and EIPA\u2010inhibitable way; this demonstrates for the first time the presence of the Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchanger in these cells. Finally, GM\u2010CSF, at doses superimposable to those needed for triggering proliferation, induces in AML\u2010193 cells a sustained alkalinization, which is dependent on a operating Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchange, as it is inhibited by EIPA. These results suggest that GM\u2010CSF, like other growth factors in other cell systems, exerts its mitogenic activity in AML\u2010193 cells by inducing a Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchanger\u2010mediated rise in pH<jats:sub>i<\/jats:sub>.<\/jats:p>","DOI":"10.1002\/jcp.1041430118","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T09:58:24Z","timestamp":1109411904000},"page":"133-139","source":"Crossref","is-referenced-by-count":4,"title":["Role of Na<sup>+<\/sup>\/H<sup>+<\/sup> exchange in the granulocyte\u2010macrophage colony\u2010stimulating factor\u2010dependent growth of a leukemic cell line"],"prefix":"10.1002","volume":"143","author":[{"given":"Daniele","family":"Caracciolo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Pannocchia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Treves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dario","family":"Ghigo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugenio","family":"Gallo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corrado","family":"Tarella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Bussolino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Turrini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giacomo","family":"Tamponi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amalia","family":"Bosia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.3.1082"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3296190"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.4049\/jimmunol.140.8.2695","article-title":"Stimulation of guanylate cyclase activity and reduction of adenylate cyclase activity by granulocyte\u2010macrophage colony\u2010stimulating factor in human blood neutrophils","volume":"140","author":"Coffey R. 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