{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T04:55:47Z","timestamp":1770526547136,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5332,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human omentum contains a potent, not yet identified angiogenic activity. The omentum is very vascularized. Therefore, we investigated whether human omental microvascular endothelial cells (HOME cells) express the angiogenic peptide basic fibroblast growth factor (bFGF). Cytosol prepared from HOME cells stimulated DNA synthesis in bovine epithelial lens cells (BEL cells). The mitogenic activity could be neutralized by an anti\u2010bFGF antibody. Basic FGF\u2010like material from the HOME cell cytosol was bound onto a heparin\u2010Sepharose column at 0.6 M and was eluted at 3 M NaCl. The 3 M NaCl eluted material reacted with the specific anti\u2010bFGF antibody in an ELISA and stimulated DNA synthesis. It did not react with a specific anti\u2010acidic fibroblast growth factor (aFGF) antibody. Western blotting experiments using the same bFGF antibody showed the presence of a major band of 17 Kd and a doublet of 20\u201022 Kd. Northern blotting of nonstimulated HOME cells using a specific 1.4 kb bFGF probe showed the presence of 5 molecular species of 6.6, 3.7, 2.2, 2.0, and 1.0kb. No aFGF mRNA was detected with a specific previously characterized 4.04 kb probe. 12\u2010O\u2010tetradecanoylphorbol 13\u2010acetate (TPA) did not influence significantly the expression of bFGF at the protein and mRNA level in HOME cells. Thus, protein kinase C activation by TPA did not appear to modulate significantly the expression of bFGF for that cell type. Contrastingly, human umbilical vein endothelial cells (HUVE cells), which expressed no bFGF and aFGF mRNA at a basal level, were induced to express bFGF but not aFGF mRNA when stimulated by TPA. These results suggest that the described angiogenic activity could be the bFGF\u2010like mitogen contained in HOME cells and that these cells are different from endothelial cells derived from large vessels (HUVE cells) regarding the expression of bFGF.<\/jats:p>","DOI":"10.1002\/jcp.1041440120","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T09:00:46Z","timestamp":1109408446000},"page":"151-158","source":"Crossref","is-referenced-by-count":50,"title":["Basic fibroblast growth factor expression in human omental microvascular endothelial cells and the effect of phorbol ester"],"prefix":"10.1002","volume":"144","author":[{"given":"A.","family":"Bikfalvi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Alterio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. L.","family":"Inyang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Dupuy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Laurent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. P.","family":"Hartmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Vigny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Raulais","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Courtois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Tobelem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80981-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2425435"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90292-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90183-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding","volume":"72","author":"Bradford M. M.","year":"1979","journal-title":"Anal. Biochem."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90281-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"9579","DOI":"10.1016\/S0021-9258(18)67551-9","article-title":"Thrombin stimulates c\u2010sis gene expression in microvascular endothelial cells","volume":"261","author":"Daniel T. O.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90306-6"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.3.679"},{"key":"e_1_2_1_11_1","article-title":"Protein kinase C\u2010mediated modulation of bFGF receptor in capillary endothelial cells. Abstract 1485","volume":"107","author":"Doctrow S. R.","year":"1989","journal-title":"Am. Soc. Cell Biol., J. Cell. Biol"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2643161"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6507"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.9.3174"},{"key":"e_1_2_1_15_1","first-page":"24","volume-title":"Angiogenesis","author":"Fiddes J.","year":"1987"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.5.1431"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(79)90038-1"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041330405"},{"key":"e_1_2_1_19_1","first-page":"579","article-title":"Increased vascular perfusion after administration of an omental lipid fraction","volume":"162","author":"Goldsmith H. S.","year":"1986","journal-title":"Surg. Gynecol. Obst."},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"2034","DOI":"10.1001\/jama.1984.03350150034017","article-title":"Lipid angiogenic factor from omentum","volume":"252","author":"Goldsmith H. S.","year":"1984","journal-title":"J. Am. Med. Assoc."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.4.1611"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.3109\/08977198809000242"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)91011-4"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107470"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110937"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)81489-8"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.14.5024"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041290220"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.7.2091"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.16.5778"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.19.7297"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-2-12-1196"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)91576-3"},{"key":"e_1_2_1_35_1","first-page":"105","article-title":"High reliable and sensitive assay for the purification of cellular growth factors","volume":"30","author":"Plouet J.","year":"1984","journal-title":"J. Cell. Mol. Biol."},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)81262-2"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041310211"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/325257a0"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(87)80001-3"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041360212"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041310312"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.8.2292"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041440120","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041440120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T15:06:10Z","timestamp":1697987170000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041440120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,7]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,7]]}},"alternative-id":["10.1002\/jcp.1041440120"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041440120","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,7]]}}}