{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:56:06Z","timestamp":1760043366443},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4967,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1991,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The plasma membranes of rat heart muscle, grown in cell culture, were made permeable with saponin in a Ca\u2010free solution. The cells were then supplied with a medium resembling the cytosol, and the adenosine triphosphate (ATP)\u2010dependent Ca<jats:sup>2+<\/jats:sup> sequestration was measured in the presence of oxalate. The nonmitochondrial component accounts for about 50% of the total Ca<jats:sup>2+<\/jats:sup> uptake. The nonmitochondrial accumulation of Ca<jats:sup>2+<\/jats:sup> within myocardial cells was found to be reversible by addition of the Ca<jats:sup>2+<\/jats:sup> ionophore A23187. On the other hand, the Ca<jats:sup>2+<\/jats:sup> antagonist D\u2010600 (50 \u03bcM) had almost no effect on Ca<jats:sup>2+<\/jats:sup> accumulation. Caffeine reduced Ca<jats:sup>2+<\/jats:sup> accumulation in the skinned cardiomyocytes in a concentration\u2010dependent manner. In addition, the anticalmodulin drug trifluo\u2010perazine (TFP) reduced Ca<jats:sup>2+<\/jats:sup> accumulation in the skinned cells. Because of the analogy between nonmitochondrial ATP\u2010dependent Ca<jats:sup>2+<\/jats:sup> accumulation and the sarcoplasmic reticulum (SR) function with regard to the influence of various agents, it is assumed that we actually measure Ca<jats:sup>2+<\/jats:sup> accumulation in the SR. The rate of Ca<jats:sup>2+<\/jats:sup> accumulation into the SR measured during the development of the cardiomyocytes in culture shows an almost linear increase as a function of culture age. Amiodarone, a potent antiarrhythmic agent, and its metabolite, desethyl\u2010amiodarone, inhibited Ca<jats:sup>2+<\/jats:sup> accumulation into SR, which may explain their therapeutic effect.<\/jats:p>","DOI":"10.1002\/jcp.1041480115","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T08:07:46Z","timestamp":1109405266000},"page":"124-132","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of sarcoplasmic reticulum in skinned heart muscle cultures"],"prefix":"10.1002","volume":"148","author":[{"given":"Leah","family":"Weinstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadassa","family":"Brik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heschi H.","family":"Rotmensch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asher","family":"Shainberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"H167","article-title":"Cardiac muscle function: Results from skinned fiber preparations","volume":"244","author":"Best P. 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