{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T05:08:17Z","timestamp":1698037697039},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4936,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1991,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Following exposure to a number of hormones, the cell membrane in Madin\u2010Darby Canine Kidney (MDCK) cells is hyperpolarized by increase of intracellular calcium activity. The present study has been performed to elucidate the possible role of calmodulin in the regulation of intracellular calcium activity and cell membrane potential. To this end trifluoperazine has been added during continuous recording of cell membrane potential or intracellular calcium. Trifluoperazine leads to a transient increase of intracellular calcium as well as a sustained hyperpolarization of the cell membrane by activation of calcium sensitive K<jats:sup>+<\/jats:sup> channels. Half\u2010maximal effects are observed between 1 and 10 \u03bcmol\/L trifluoperazine. A further calmodulin antagonist, chlorpromazine, (50 \u03bcmol\/L), similarly hyperpolarizes the cell membrane. The effects of trifluoperazine are virtually abolished in the absence of extracellular calcium. Pretreatment of the cells with either pertussis toxin or phorbolester TPA does not interfere with the hyperpolarizing effect of trifluoperazine. In conclusion, calmodulin is apparently involved in the regulation of calcium transfer across the cell membrane but not in the stimulation of K<jats:sup>+<\/jats:sup> channels by intracellular calcium.<\/jats:p>","DOI":"10.1002\/jcp.1041480218","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T08:17:57Z","timestamp":1109405877000},"page":"314-319","source":"Crossref","is-referenced-by-count":2,"title":["Effect of trifluoperazine on renal epitheloid madin\u2010darby canine kidney cells"],"prefix":"10.1002","volume":"148","author":[{"given":"M.","family":"Paulmichl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"W\u00f6ll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Waldegger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Lang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(85)80033-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(89)90168-9"},{"key":"e_1_2_1_4_1","first-page":"195","article-title":"Phenothiazines and haloperidol block Ca\u2010activated K channels in rat forebrain synaptosomes","volume":"33","author":"Benishin C. 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