{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:56:09Z","timestamp":1790690169848,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4905,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1991,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previously we described a cell line OCI\u2010LY3 derived from a patient with non\u2010Hodgkin's lymphoma. The cell line produced interleukin\u20106 (IL\u20106) mRNA and protein and demonstrated an autocrine pattern of growth for IL\u20106. Southern blot analysis of the IL\u20106 gene did not reveal any rearrangement. To determine whether the production of IL\u20106 by OCI\u2010LY3 was due to subtle changes in the promoter of IL\u20106 or due to the expression of trans\u2010acting factors chloramphenicol acetyltransferase (CAT) reporter constructs containing from \u20101, 180 to + 13 to \u2010112 to + 13 of a normal IL\u20106 gene were elecroporated into the cell line. When these constructs are transferred into unstimulated fibroblasts, no CAT activity is seen; however, CAT activity is induced when the cells are stimulated with either IL\u20101\u03b1, lipopolysaccharide (LPS), or cyclic adenosine monophosphate (cAMP) analogues. When the cell line OCI\u2010LY3 was transfected with these constructs, CAT activity was observed; it was not necessary to stimulate the cells with exogenous factors to observe this activity. No CAT activity was observed in a second lymphoma cell line, OCI\u2010LY13.1, that does not produce IL\u20106. These results suggest that the constitutive production of IL\u20106 by the cell line OCI\u2010LY3 is due to the presence of trans\u2010acting factors that stimulate the expression of IL\u20106 and not due to a <jats:italic>cis<\/jats:italic>\u2010acting mutation of the IL\u20106 promoter.<\/jats:p>","DOI":"10.1002\/jcp.1041480314","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T08:07:51Z","timestamp":1109405271000},"page":"426-429","source":"Crossref","is-referenced-by-count":6,"title":["Regulation of interleukin\u20106 expression in the lymphoma cell line OCI\u2010LY3"],"prefix":"10.1002","volume":"148","author":[{"given":"C. S.","family":"Yee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. A.","family":"Messner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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S.","year":"1989","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"3875","DOI":"10.4049\/jimmunol.141.11.3875","article-title":"Genomic structure of the murine IL\u20106 gene: high degree conservation of potential regulatory sequences between mouse and human","volume":"141","author":"Tanabe O.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1182\/blood.V74.2.572.572","article-title":"Production of growth factors by malignant lymphoma cell lines","volume":"74","author":"Tweeddale M.","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1182\/blood.V69.5.1307.1307","article-title":"The presence of clonogenic cells in high grade malignant lymphoma: a prognostic factor","volume":"69","author":"Tweeddale M. 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