{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T19:17:35Z","timestamp":1771010255583,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4875,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1991,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have shown that treatment of human myeloid leukemia cells with 12\u2010O\u2010tetradecanoylphorbol\u201013\u2010acetate (TPA) is associated with induction of monocytic differentiation and expression of the c\u2010jun and c\u2010fos early response genes. The present work demonstrates that the glucocorticoid dexamethasone inhibits TPA\u2010induced increases in c\u2010jun and c\u2010fos mRNA levels in U\u2010937 leukemia cells. These findings were associated with a block in appearance of the monocytic phenotype, including inhibition of TPA\u2010induced increases in lamin A, lamin C, and vimentin transcripts. Other studies have demonstrated that TPA\u2010induced monocytic differentiation and expression of the c\u2010jun and c\u2010fos genes in myeloid leukemia cells are regulated by protein kinase C (PKC). The finding that dexamethasone has no effect on TPA\u2010induced activation of PKC suggests that this glucocorticoid inhibits signals downstream or parallel to this enzyme. Nuclear run\u2010on assays demonstrate that: (1) induction of c\u2010jun and c\u2010fos expression by TPA is regulated by transcriptional mechanisms, (2) TPA\u2010induced expression of c\u2010jun and c\u2010fos does not require protein synthesis, and (3) TPA\u2010induced expression of both genes is inhibited at the transcriptional level by dexamethasone. To further define the effects of dexamethasone at the molecular level, we prepared a series of deleted c\u2010jun promoter fragments linked to the chloramphenicol acetyltransferase (CAT) gene. Increases in CAT activity during transient expression of these constructs in TPA\u2010treated U\u2010937 cells could be assigned to the region (\u221297 to \u221220) of the promoter that contains the AP\u20101 binding site. This induction of CAT activity was sensitive to dexamethasone. These findings suggest that dexamethasone down\u2010regulates TPA\u2010induced transcription of the c\u2010jun gene during monocytic differentiation by inhibiting activation of the AP\u20101 site.<\/jats:p>","DOI":"10.1002\/jcp.1041490116","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T08:18:00Z","timestamp":1109405880000},"page":"125-131","source":"Crossref","is-referenced-by-count":21,"title":["Inhibition of phorbol ester\u2010induced monocytic differentiation by dexamethasone is associated with down\u2010regulation of c\u2010fos and c\u2010jun (AP\u20101)"],"prefix":"10.1002","volume":"149","author":[{"given":"Ralf","family":"Hass","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Brach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surender","family":"Kharbanda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcnter","family":"Giese","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Traub","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald","family":"Kufe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90143-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/332166a0"},{"key":"e_1_2_1_4_1","first-page":"5106","article-title":"Induction of cytoskeleton\u2010associated proteins during differentiation of human myeloid leukemic cell lines","volume":"42","author":"Bernal S. 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