{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:39:48Z","timestamp":1698212388502},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4814,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1991,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the present study, we investigated the role of intracellular Ca++ in the stimulation of the Na+\/K+\/Cl\u2013 cotransport in synchronized BALB\/c 3T3 cells. The Na+\/K+\/Cl\u2013 cotransport was stimulated by the growth factors EGF, TGF\u2010\u03b1, IGF\u20101, and IGF\u20102, which do not activate protein kinase C, but do induce a transient increase in free cytoplasmic Ca++ In addition, direct activation of protein kinase C by the phorbol ester 12\u2010O\u2010tetradecanoyl\u2010phorbol\u201013\u2010acetate (TPA) did not affect the Na+\/K+\/Cl\u2013 cotransport activity of quiescent cells. The Na+\/K+\/Cl\u2013 cotransport was also stimulated by the above mitogens in cells pretreated with the phorbol ester TPA. This treatment led to a progressive decline in the activity of cellular protein kinase C. This result implies that cells deficient in protein kinase C may still support stimulation of the Na+\/K+\/Cl\u2013 cotransport. Taken as a whole, these findings suggest that the Na+\/K+\/Cl\u2013 cotransport is stimulated predominantly by a protein kinase C\u2010independent mechanism in BALB\/c 3T3 fibroblasts.<\/jats:p><jats:p>Both the intracellular Ca++ antagonist 8\u2010(N,N\u2010diethylamino)octyl\u20103,4,5\u2010trimethoxybenzoate (TMB\u20108) and two potent calmodulin antagonists, trifluoperazine (TFP) and chloropromazine(CP), blocked serum\u2010 and mitogen\u2010stimulated Na+\/K+\/Cl\u2013 cotransport. These results suggest that the Na+\/K+\/Cl\u2013 cotransport is stimulated by an increase of intracellular Ca++ and subsequently by a Ca++\u2010calmodulin\u2010mediated pathway in the synchronized BALB\/c 3T3 fibroblasts.<\/jats:p>","DOI":"10.1002\/jcp.1041490320","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T08:30:21Z","timestamp":1109406621000},"page":"497-502","source":"Crossref","is-referenced-by-count":7,"title":["Na+\/K+\/Cl\u2212 cotransport is stimulated by a Ca++\u2010calmodulin\u2013;mediated pathway in BALB\/c 3T3 fibroblasts"],"prefix":"10.1002","volume":"149","author":[{"given":"David","family":"Snyder","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henri","family":"Atlan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Markus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rivka","family":"Panet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041230216"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0021-9258(17)36153-7","article-title":"Lack of association of epidermal growth factor\u2010, insulin\u2010, and serum\u2010induced mitogenesis with stimulation of phosphoinositide degradation in BALB\/c 3T3 fibroblasts","volume":"261","author":"Besterman J. M.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_4_1","first-page":"13304","article-title":"Growth factor\u2010stimulated protein phosphorylation in 3T3\u2010L1 cells","volume":"260","author":"Blackshear P. J.","year":"1985","journal-title":"Evidence for protein kinase C\u2010dependent and independent pathways. J. Biol. Chem."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90029-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"6667","DOI":"10.1016\/S0021-9258(18)83479-2","article-title":"Regulation of Na+\/K+\/Cl\u2010 cotransport and [3H]bumetanme binding site density by phorbol esters in HT29 cells","volume":"264","author":"Franklin C. C.","year":"1989","journal-title":"J. Biol. Chem"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"10380","DOI":"10.1016\/S0021-9258(18)67535-0","article-title":"Effect of mitogens on ornithine decarboxylase activity and messenger RNA levels in normal and protein kinase C\u2010deficient NIH\u20103T3 fibroblasts","volume":"261","author":"Hovis J. G.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_8_1","first-page":"454","article-title":"Selective binding of antipsychotics and and other psychoactive agents to the calcium\u2010dependent activator of cyclic nucleotide phosphodiesterase","volume":"208","author":"Levin R. M.","year":"1979","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(74)90198-8"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"8378","DOI":"10.1016\/S0021-9258(18)43501-6","article-title":"Inhibitory action of chloropromazine, dibucaine, and other phospho\u2010lipid\u2010interacting drugs on calcium\u2010activated, phospholipid\u2010dependent protein kinase","volume":"255","author":"Mori","year":"1980","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/308693a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041320216"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.14.4334"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.2.454"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.11.3537"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(82)92005-8"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041170105"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1985.248.3.C288"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(85)90355-4"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041450106"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870226"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01871952"},{"key":"e_1_2_1_23_1","first-page":"117","volume":"859","author":"Panet R.","year":"1986","journal-title":"Acta"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2390745"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041400119"},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1016\/S0021-9258(19)84545-3","article-title":"Growth factors activate the bumetanide\u2010sensitive Na+\/K+ \/Cl\u2010 cotransport in hamster fibroblasts","volume":"261","author":"Paris S.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(84)80213-2"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3018928"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041460318"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.6.2316"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040910313"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90467-X"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90466-8"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.8.2384"},{"key":"e_1_2_1_35_1","first-page":"21","article-title":"Calcium and calmodulin in cell growth and transformation","volume":"735","author":"Veigl M. L.","year":"1984","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.23.8053"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041490320","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041490320","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T04:46:11Z","timestamp":1698036371000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041490320"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,12]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,12]]}},"alternative-id":["10.1002\/jcp.1041490320"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041490320","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,12]]}}}