{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:23:08Z","timestamp":1770337388387,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4814,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1991,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of albumin binding to cultured bovine pulmonary artery endothelial cell (BPAEC) monolayers on the transendothelial flux of <jats:sup>125<\/jats:sup>I\u2010labelled bovine serum albumin (BSA) was examined to determine its possible role on albumin transcy\u2010tosis. The transport of <jats:sup>125<\/jats:sup>I\u2010BSA tracer across BPAEC grown on gelatin\u2010 and fibronectin\u2010coated filters (0.8 \u03bcm pore diam.) was affected by the presence of unlabelled BSA in the medium in that transendothelial <jats:sup>125<\/jats:sup>I\u2010BSA permeability decreased, reaching a 40% reduction at BSA concentrations equal to or greater than 5 mg\/ml. BSA binding to BPAEC monolayers was saturated at concentration of 10 mg\/ml with an apparent binding affinity of 6 x 10<jats:sup>\u22127<\/jats:sup> M. In contrast, gelatin added to the medium altered neither <jats:sup>125<\/jats:sup>I\u2010BSA binding nor transport. Several lectins were tested for their ability to inhibit <jats:sup>125<\/jats:sup>I\u2010BSA binding and transport. One lectin, <jats:italic>Ricinus communis<\/jats:italic> (RCA), reduced <jats:sup>125<\/jats:sup>I\u2010BSA binding by 70% and transport by 40%. Other lectins, <jats:italic>Ulex europaeus, Triticum vulgare<\/jats:italic>, and <jats:italic>Glycine max<\/jats:italic> decreased neither <jats:sup>125<\/jats:sup>I\u2010BSA binding nor transport. The reduction of <jats:sup>125<\/jats:sup>I\u2010BSA transport by RCA was not observed in the presence of saturating levels of BSA, indicating that RCA influenced only the albumin\u2010dependent component of transport. RCA, but not other lectins, precipitated a 60 kDa plasmalemmal glycoprotein from cell lysates of surface radioiodinated BPAEC monolayers. This 60 kDa glycoprotein appears to be the equivalent of gp60 identified previously as an albumin binding glycoprotein in rat microvascular endothelium. In summary, approximately 40% of albumin transport across' BPAEC monolayers is dependent on albumin binding. This component of albumin transport is inhibited by 80% by the binding of RCA to gp60. These results suggest that binding of albumin to gp60 on pulmonary artery endothelial cell membrane is a critical determinant of transendothelial albumin flux involving mechanisms such as plasmalemmal vesicular transcytosis.<\/jats:p>","DOI":"10.1002\/jcp.1041490329","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T08:30:21Z","timestamp":1109406621000},"page":"575-584","source":"Crossref","is-referenced-by-count":32,"title":["Lectin binding to gp60 decreases specific albumin binding and transport in pulmonary artery endothelial monolayers"],"prefix":"10.1002","volume":"149","author":[{"given":"Alma","family":"Siflinger\u2010Birnboim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Schnitzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazel","family":"Lum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank A.","family":"Blumenstock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien\u2010Ping J.","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Del Vecchio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asrar B.","family":"Malik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-708X(64)90071-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1152\/jappl.1987.62.3.1076","article-title":"Measurement of albumin permeability across endothelial monolayers in vitro","volume":"62","author":"Cooper J. A.","year":"1987","journal-title":"J. Appl. Physiol."},{"key":"e_1_2_1_4_1","first-page":"67","volume-title":"The Endothelial Cell\u2013A Pluripotent Control Cell of the Vessel Wall","author":"Del Vecchio P. J.","year":"1983"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(78)91322-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041280115"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)83652-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.1.231"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.4.1304"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-708X(82)90144-2"},{"key":"e_1_2_1_11_1","first-page":"60","article-title":"Ulex europaeus I Lectin as a marker for vascular endothelium in human tissues","volume":"47","author":"Holth\u00f6fer H.","year":"1982","journal-title":"Lab. Invest."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/312162a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3883490"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.6.2603"},{"key":"e_1_2_1_15_1","first-page":"1158","article-title":"Factors influencing DTPA conjugation with antibodies by cyclic DTPA anhydride","volume":"24","author":"Paik C. H.","year":"1983","journal-title":"J. Nucl. Med."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/36.5.3356897"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"H425","DOI":"10.1152\/ajpheart.1988.254.3.H425","article-title":"Specific albumin binding to microvascular endothelium in culture","volume":"254","author":"Schnitzer J. E.","year":"1988","journal-title":"Am. J. Physiol. (Heart Circ. Physiol. 23)"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.18.6773"},{"key":"e_1_2_1_19_1","first-page":"241","article-title":"Lectin analysis of common glycoproteins detected on the surface of continuous microvascular endothelium in situ and in culture: Identification of sialoglycoproteins","volume":"52","author":"Schnitzer J. E.","year":"1990","journal-title":"Eur. J. Cell Biol."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.17.6843"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041320115"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"577","DOI":"10.2220\/biomedres.4.577","article-title":"Immunocytochemical evidence for transendothelial transport of albumin and fibrinogen in rat heart and diaphragm","volume":"4","author":"Yokota S.","year":"1983","journal-title":"Biomed. Res."}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041490329","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041490329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T04:46:55Z","timestamp":1698036415000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041490329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,12]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,12]]}},"alternative-id":["10.1002\/jcp.1041490329"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041490329","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,12]]}}}