{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:50:56Z","timestamp":1791121856308,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,9,16]],"date-time":"2003-09-16T00:00:00Z","timestamp":1063670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"The Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["#MOP-42400"],"award-info":[{"award-number":["#MOP-42400"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fondation de l'H\u00f4pital du Saint-Sacrement"},{"name":"Centre de recherche du CHA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    During wound healing, myofibroblasts play a central role in matrix formation and wound contraction. At the end of healing, there is evidence that myofibroblasts disappear via apoptotic pathways. Hypertrophic scars are a fibroproliferative disorder that leads to considerable morbidity. It has been postulated that a defect in myofibroblast apoptosis could be responsible for the pathological scar formation, but no evidence exists. We have isolated and cultured human normal wound (Wmyo) and hypertrophic scar (Hmyo) myofibroblasts and compared their basal apoptotic rates and their sensitivity to serum starvation and Fas antibody\u2010induced apoptosis to that obtained for dermal fibroblasts (Fb). A higher rate of apoptosis as evidenced by morphological criteria and a propidium iodide assay was observed for Wmyo in comparison to Fb and Hmyo. These results came along with a low level of the anti\u2010apoptotic proteins Bcl\u20102 and Bclx\n                    <jats:sub>L<\/jats:sub>\n                    in Wmyo, whereas there was an increase in the level of the pro\u2010apoptotic molecule Bax when compared to the results obtained for Fb and Hmyo. Hmyo showed a higher level of Bcl\u20102 compared to Fb but no difference in the Bax or Bclx\n                    <jats:sub>L<\/jats:sub>\n                    level. After serum starvation, Wmyo revealed an increased apoptotic rate, whereas Hmyo and Fb did not show any difference. Anti\u2010Fas treatment did not modify the levels of apoptosis but strongly increased the cell growth of Hmyo as compared to Wmyo. This is the first study presenting a broad vision of the apoptotic sensitivity of normal and pathological myofibroblasts. These results confirmed the hypothesis of defects in apoptosis and growth during pathological scar formation impeding myofibroblast disappearance at the end of healing.  J. Cell. Physiol. 198: 350\u2013358, 2004\u00a9 2003 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/jcp.10415","type":"journal-article","created":{"date-parts":[[2004,1,27]],"date-time":"2004-01-27T06:31:40Z","timestamp":1075185100000},"page":"350-358","source":"Crossref","is-referenced-by-count":118,"title":["Normal skin wound and hypertrophic scar myofibroblasts have differential responses to apoptotic inductors"],"prefix":"10.1002","volume":"198","author":[{"given":"V\u00e9ronique","family":"Moulin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Larochelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Langlois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Thibault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Lopez\u2010Vall\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,9,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00339-B"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780039"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-475x.2001.00501.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400605"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64803-1"},{"key":"e_1_2_6_7_1","first-page":"21","article-title":"A smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing","volume":"63","author":"Darby I","year":"1990","journal-title":"Lab Invest"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(96)00117-3"},{"key":"e_1_2_6_9_1","first-page":"56","article-title":"Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar","volume":"146","author":"Desmouli\u00e8re A","year":"1995","journal-title":"Am J Pathol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-475X.1996.40206.x"},{"key":"e_1_2_6_11_1","first-page":"105","article-title":"Morphological and immunochemical differences between keloid and hypertrophic scar","volume":"145","author":"Ehrlich HP","year":"1994","journal-title":"Am J Pathol"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12334273"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.76.3.561"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1994.1143"},{"key":"e_1_2_6_15_1","first-page":"465","article-title":"Enhanced expression of mRNA for transforming growth factor\u2010b, type I and type III procollagen in human post\u2010burn hypertrophic scar tissues","volume":"122","author":"Ghahary A","year":"1993","journal-title":"J Lab Clin Med"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1004058010500"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1995.1074"},{"key":"e_1_2_6_18_1","first-page":"16","article-title":"Defining apoptosis","volume":"146","author":"Hockenbery D","year":"1995","journal-title":"Am J Pathol"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199804)175:1<19::AID-JCP3>3.0.CO;2-F"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1242\/jcs.107.5.1169","article-title":"Serum deprivation induces apoptotic cell death in a subset of Balb\/c 3T3 fibroblasts","volume":"107","author":"Kulkarni G","year":"1994","journal-title":"J Cell Sci"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.33.20490"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.4.901"},{"key":"e_1_2_6_23_1","first-page":"237","article-title":"Regulation of radiation\u2010induced apoptosis in oncogene\u2010transfected fibroblasts: Influence of H\u2010ras on the G2 delay","volume":"12","author":"McKenna W","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_6_24_1","first-page":"1","article-title":"Growth factors in skin wound healing","volume":"68","author":"Moulin V","year":"1995","journal-title":"Eur J Cell Biol"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04975.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-4179(95)00167-0"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1997.3827"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-58456-5_13"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1110"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1067\/msy.2001.116453"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00371800"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/24055"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02624473"},{"key":"e_1_2_6_34_1","first-page":"850","volume-title":"Biostatistique","author":"Scherrer B","year":"1984"},{"key":"e_1_2_6_35_1","first-page":"109","volume-title":"Histology for pathologists","author":"Sch\u00fcrch W","year":"1992"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202966"},{"key":"e_1_2_6_37_1","first-page":"4119","article-title":"Expression level of Bcl\u20102 determines anti\u2010 or proapoptotic function","volume":"59","author":"Shinoura N","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_6_38_1","first-page":"532","article-title":"Comparative growth dynamics and actin concentration between cultured human myofibroblasts from granulating wounds and dermal fibroblasts from normal skin","volume":"61","author":"Vande Berg JS","year":"1989","journal-title":"Lab Invest"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.10415","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.10415","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T07:17:30Z","timestamp":1696663050000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.10415"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9,16]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,3]]}},"alternative-id":["10.1002\/jcp.10415"],"URL":"https:\/\/doi.org\/10.1002\/jcp.10415","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9,16]]}}}