{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:09:10Z","timestamp":1758708550383},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4692,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1992,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We compared the pattern of K<jats:sup>+<\/jats:sup> channels and the mitogenic sensitivity to K<jats:sup>+<\/jats:sup> channel blocking agents in primary cultures of rabbit proximal tubule cells (PC.RC) (Ronco et al., 1990) and two derived SV\u201040\u2010transformed cell lines exhibiting specific functions of proximal (RC.SV1) and more distal (RC.SV2) tubule cells (Vandewalle et al., 1989). First, K<jats:sup>+<\/jats:sup> channel equipment surveyed by the patchclamp technique was modified after SV\u201040 transformation in both cell lines; although a high conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> channel [K<jats:sup>+<\/jats:sup><jats:sub>200<\/jats:sub> (Ca<jats:sup>2+<\/jats:sup>)] remained the most frequently recorded K<jats:sup>+<\/jats:sup> channel, the transformed state was characterized by emergence of three Ca<jats:sup>2+<\/jats:sup>\u2010insensitive K<jats:sup>+<\/jats:sup> channels (150, 50, and 30 pS), virtually absent from primary culture, contrasting with reduced frequency of two Ca<jats:sup>2+<\/jats:sup>\u2010sensitive K<jats:sup>+<\/jats:sup> channels (80 and 40 pS). Second, quinine (Q), tetraethylammonium ion (TEA) and charybdotoxin (CTX), at concentrations not affecting cell viability, all decreased <jats:sup>3<\/jats:sup>H\u2010TdR incorporation and cell growth in PC.RC cultures, but only TEA had similar effects in transformed cells. The latter were further characterized by paradoxical effects of Q that induced a marked increase in thymidine incorporation. Q also exerted contrasting effects on channel activity: it inhibited the [K<jats:sup>+<\/jats:sup><jats:sub>200<\/jats:sub> (Ca<jats:sup>2+<\/jats:sup>)] when the channel was highly active, with a K<jats:sub>i<\/jats:sub> (0.2 mM) similar to that measured for <jats:sup>3<\/jats:sup>H\u2010TdR incorporation in PC.RC cells (0.3 mM), but increased the mean current through poorly active channels. TEA blocked all K<jats:sup>+<\/jats:sup> channels with conductance \u2265 50 pS, including the [K<jats:sup>+<\/jats:sup><jats:sub>200<\/jats:sub> (Ca<jats:sup>2+<\/jats:sup>)], in a range of concentrations that substantially affected cell proliferation. The unique effect of TEA on SV\u201040\u2010transformed cells might be related to broad inhibition of K<jats:sup>+<\/jats:sup> channels. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041510116","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T07:28:12Z","timestamp":1109402892000},"page":"113-125","source":"Crossref","is-referenced-by-count":27,"title":["Transformation of renal tubule epithelial cells by simian virus\u201040 is associated with emergence of Ca<sup>2+<\/sup>\u2010insensitive K<sup>+<\/sup> channels and altered mitogenic sensitivity to K<sup>+<\/sup> channel blockers"],"prefix":"10.1002","volume":"151","author":[{"given":"Jacques","family":"Teulon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre M.","family":"Ronco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique","family":"Geniteau\u2010Legendre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e9atrice","family":"Baudouin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Estrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Cassingena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Vandewalle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.4049\/jimmunol.144.6.2038","article-title":"Ion channel blockers inhibit B cell activation at a precise stage of the G1 phase of the cell cycle","volume":"144","author":"Amigorena S.","year":"1990","journal-title":"J. Immunol."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.91.3.317"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041320302"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.84.1.1"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869613"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041130107"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2437651"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2449730"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/3.2.159"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.1.318"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/307465a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.89.3.405"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016016"},{"key":"e_1_2_1_15_1","first-page":"F458","article-title":"Ca\u2010activated K+ channels in apical membrane of mammalian CCT, and their role in K+ secretion","volume":"252","author":"Frindt G.","year":"1987","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_16_1","first-page":"8","article-title":"Ion channels in mammalian proximal renal tubules","volume":"13","author":"G\u00f6gelein H.","year":"1990","journal-title":"Renal Physiol. Biochem."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1989.69.2.417"},{"key":"e_1_2_1_18_1","first-page":"74","article-title":"Na+ \/H+ exchange and growth factor\u2010induced cytosolic pH changes. Role in cellular proliferation","volume":"85","author":"Grinstein S.","year":"1989","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1987.252.2.C128"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00656997"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.90.1.27"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041410126"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870671"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.4.1200"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2448877"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869631"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.20.6275"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90156-0"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/343764a0"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/313316a0"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017656"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1987.67.3.1054"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.24.10171"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/219198a0"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1991.260.1.C104"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041450206"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3018928"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.93.6.1061"},{"key":"e_1_2_1_40_1","doi-asserted-by":"crossref","first-page":"3224","DOI":"10.4049\/jimmunol.139.10.3224","article-title":"The inhibitory effects of K+ channel\u2010blocking agents on T lymphocyte proliferation and lymphokine production are \u201cnonspecific\u201d","volume":"139","author":"Schell S. R.","year":"1987","journal-title":"J. Immunol."},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(71)90725-8"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.259.1.C56"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.11.5560"},{"key":"e_1_2_1_44_1","doi-asserted-by":"crossref","first-page":"14607","DOI":"10.1016\/S0021-9258(18)66914-5","article-title":"Purification of charybdotoxin, a specific inhibitor of the high\u2010conductance Ca2+\u2010activated K+ channel","volume":"261","author":"Smith C.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_45_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1182\/blood.V56.2.315.315","article-title":"Suppression and potentiation of mouse hemopoietic progenitor cell proliferation by ouabain","volume":"56","author":"Spivak J. L.","year":"1980","journal-title":"Blood"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041340103"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041410128"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90187-J"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041510116","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041510116","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T16:13:43Z","timestamp":1695831223000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041510116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,4]]}},"alternative-id":["10.1002\/jcp.1041510116"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041510116","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4]]}}}