{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:09:06Z","timestamp":1790478546592,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4601,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1992,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leukemia inhibitory factor\/differentiation\u2010stimulating factor (LIF\/D\u2010factor), expression of its mRNA, and possible roles in bone metabolism were studied in murine primary and clonal osteoblast\u2010like cells. Local bone\u2010resorbing factors such as IL\u20101, TNF\u03b1, and LPS strongly induced expression of LIF\/D\u2010factor mRNA in both clonal MC3T3\u2010E1 cells and primary osteoblast\u2010like cells. Neither parathyroid hormone nor 1\u03b1,25\u2010dihydroxyvitamin D<jats:sub>3<\/jats:sub> stimulated expression of LIF\/D\u2010factor mRNA. LIF\/D\u2010factor per se did not stimulate expression of its own mRNA.<\/jats:p><jats:p>Appreciable amounts of LIF\/D\u2010factor were detected in synovial fluids from rheumatoid arthritis (RA) patients but not in those with osteoarthritis (OA). Simultaneous treatment with LIF\/D\u2010factor, IL\u20101, and IL\u20106 at the concentrations found in synovial fluids from RA patients greatly enhanced bone resorption, though these cytokines did not stimulate bone resorption when separately applied. This suggests that LIF\/D\u2010factor produced by osteoblasts is in concert with other boneresorbing cytokines such as IL\u20101 and IL\u20106 involved in the bone resorption seen in the joints of RA patients.<\/jats:p><jats:p>LIF\/D\u2010factor specifically bound to MC3T3\u2010E1 cells with an apparent dissociation constant of 161 pM and 1,100 binding sites\/cell. LIF\/D\u2010factor dose\u2010dependently suppressed incorporation of [<jats:sup>3<\/jats:sup>H]thymidine into MC3T3\u2010E1 cells. In addition, it potentiated the alkaline phosphatase activity induced by retinoic acid, though LIF\/D\u2010factor alone had no effect on enzyme activity. These results suggest that LIF\/D\u2010factor is involved in not only osteoclastic bone resorption but also osteoblast differentiation in conjugation with other osteotropic factors. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041520110","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:45:46Z","timestamp":1109400346000},"page":"71-78","source":"Crossref","is-referenced-by-count":59,"title":["Leukemia inhibitory factor\/differentiation\u2010stimulating factor (LIF\/D\u2010factor): Regulation of its production and possible roles in bone metabolism"],"prefix":"10.1002","volume":"152","author":[{"given":"Yoshiko","family":"Ishimi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etsuko","family":"Abe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng He","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chisato","family":"Miyaura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei Hua","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Midori","family":"Oshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisashi","family":"Kurosawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikio","family":"Tomida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoo","family":"Hozumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuo","family":"Suda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.16.5958"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650030609"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041450116"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.4049\/jimmunol.143.4.1163","article-title":"Hepatocyte\u2010stimulating factor III shares structural and functional identity with leukemia\u2010inhibitory factor","volume":"143","author":"Baumann H.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_6_1","first-page":"100","volume-title":"Perspectives in Rheumatology 1987: Current Medical Literature","author":"Buchan G.","year":"1987"},{"key":"e_1_2_1_7_1","first-page":"449","article-title":"Interleukin\u20101 and tumor necrosis factor mRNA expression in rheumatoid arthritis: Prolonged production of IL\u20101\u03b1","volume":"73","author":"Buchan G.","year":"1988","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780300804"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00541245"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02742.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07833.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240460105"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.16.5490"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830181122"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040760207"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.145.10.3297"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1.1989.8.351"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)71242-8"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.15.5948"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/336690a0"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-127-1-185"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.4049\/jimmunol.140.1.112","article-title":"Synergistic interactions of interleukin 1, interferon\u2010\u03b3 and tumor necrosis factor in terminally differentiating a mouse myeloid leukemic cell line (M1)","volume":"140","author":"Onozaki K.","year":"1988","journal-title":"J. Immunol."},{"key":"e_1_2_1_23_1","first-page":"3602","article-title":"Synergistic regulatory effects of interleukin 6 and interleukin 1 on the growth and differentiation of human and mouse myeloid leukemic cell lines","volume":"49","author":"Onozaki K.","year":"1989","journal-title":"Cancer Res."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2783498"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-126-3-1416"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-127-4-1602"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02555737"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)90577-2"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/336688a0"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/336684a0"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.16.7298"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2512641"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1989.tb02277.x"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041520110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041520110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T01:34:49Z","timestamp":1698111289000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041520110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,7]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,7]]}},"alternative-id":["10.1002\/jcp.1041520110"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041520110","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,7]]}}}