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Bethanechol similarly decreases intracellular pH in isolated cells suspended in bicarbonate\u2010free buffer in a time course similar to inhibition of enterocyte Na uptake, suggesting inhibition of Na\/H exchange. In brush border membrane vesicles rapidly prepared from cells stimulated with bethanechol, proton\u2010dependent <jats:sup>22<\/jats:sup>Na uptake is transiently inhibited in a time course similar to inhibition of cell Na uptake. Bethanechol also stimulates transient translocation of protein kinase C from the cytosol to the particulate fraction, a portion of this activity translocating to the brush border membrane. To determine the calcium dependence of bethanechol's action, enterocytes were loaded with varying concentrations of the calcium buffering agent quin\u20102. Inhibition of cell Na uptake by the calcium ionophore ionomycin could be completely reversed by quin\u20102 buffering in a concentration\u2010dependent manner. In contrast, quin\u20102 buffering had little or no effect on the inhibition of Na uptake caused by the protein kinase C activators phorbol esters and oleoylacetylglycerol. Bethanechol's inhibitory effects were partially, but not completely reversed by quin\u20102 buffering. These data suggest that the effects of bethanechol on chicken villus enterocyte brush border Na\/H exchange are mediated by calcium\u2010dependent process(es) as well as by protein kinase C. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041520218","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:55:31Z","timestamp":1109400931000},"page":"362-371","source":"Crossref","is-referenced-by-count":7,"title":["Bethanechol inhibition of chicken intestinal brush border Na\/H exchange: Role of protein kinase C and other calcium\u2010dependent processes"],"prefix":"10.1002","volume":"152","author":[{"given":"Mark W.","family":"Musch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna","family":"Drabik\u2010Arvans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mrinalini C.","family":"Rao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene B.","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.001111"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.242.5.C404"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_5_1","first-page":"G99","article-title":"Carbachol\u2010induced protein phosphorylation in parietal cells: Regulation by [Cai]","volume":"257","author":"Brown M. 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