{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:24:35Z","timestamp":1760707475847},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4539,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1992,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We examined the role of the factor deficient in xeroderma pigmentosum group A (XP\u2010A) cells in the formation of proliferating cell nuclear antigen (PCNA) complex with DNA in the DNA repair process in human fibroblasts following cisdiamminedichloroplatinum (CDDP)\u2010treatment. Immunofluorescence staining after methanol fixation was used to detect the PCNA complex formation. When quiescent normal cells were PCNA\u2010stained at 3 h after 100 \u03bcM CDDP treatment for 1 h, almost all nuclei of the cells showed a punctuated staining pattern. On the other hand, nuclei of XP\u2010A cells were not stained. These results were the same with the findings following 10J\/m<jats:sup>2<\/jats:sup> of ultraviolet light (UV)\u2010irradiation. The quantitative analysis of the PCNA immunofluorescence intensity of normal cells revealed that the mean intensity was increased by 4.8 times by the CDDP\u2010treatment and 6.1 times by the UV\u2010irradiation, compared with that of untreated cells. The intensities among nuclei ranged widely in both treatments. In contrast, the mean intensity was not increased in XP\u2010A cells by the same treatments. However, when XP\u2010A cells were fused with normal cells with polyethylene glycol (PEG) treatment, the nuclei of the XP\u2010A cells showed positive PCNA\u2010staining following CDDP\u2010treatment or UV\u2010irradiation in almost all cases. These results suggest that the PCNA complex formation may play a role in the DNA repair process after the step where the factor deficient in XP\u2010A cells is involved following CDDP\u2010treatment as well as following UV\u2010irradiation. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041520324","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:58:08Z","timestamp":1109401088000},"page":"639-645","source":"Crossref","is-referenced-by-count":13,"title":["Restoration of proliferating cell nuclear antigen (PCNA) complex formation in xeroderma pigmentosum group a cells following cis\u2010diamminedichloroplatinum (II)\u2010treatment by cell fusion with normal cells"],"prefix":"10.1002","volume":"152","author":[{"given":"Masahiko","family":"Miura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaharu","family":"Domon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehito","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinari","family":"Takasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/326515a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)81127-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/218652a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.63.2.428"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.20.8073"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4781(83)90107-0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(90)90076-H"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2897717"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(82)90163-X"},{"key":"e_1_2_1_11_1","first-page":"5713","article-title":"Increased DNA repair as a mechanism of acquired resistance to cis\u2010diamminedichloroplatinum (II) in human ovarian cancer cell lines","volume":"48","author":"Masuda H.","year":"1988","journal-title":"Cancer Res."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(81)90467-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041500221"},{"key":"e_1_2_1_14_1","first-page":"4178","article-title":"Formation and repair of DNA interstrand cross\u2010links in relation to cytotoxicity and unscheduled DNA synthesis induced in control and mutant human cells treated with cis\u2010diamminedichloroplatinum (II)","volume":"45","author":"Plooy A. 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