{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:50:26Z","timestamp":1761058226730},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4509,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mechanism by which <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> (used as a tracer for K<jats:sup>+<\/jats:sup>) enters human nonpigmented ciliary epithelial cells were investigated. Ouabain\u2010inhibitable bumetanide\u2010insensitive <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> transport accounted for \u223c70\u201380% of total, whereas bumetanide\u2010inhibitable ouabain\u2010insensitive uptake accounted for 15\u201325% of total. K<jats:sup>+<\/jats:sup> channel blockers such as BaCl<jats:sub>2<\/jats:sub> reduced uptake by \u223c5%. Bumetanide inhibited <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake with an IC<jats:sub>50<\/jats:sub> of 0.5 \u03bcM, while furosemide inhibited with an IC<jats:sub>50<\/jats:sub> of about 20 \u03bcM. Bumetanide\u2010inhibitable <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake was reduced in Na<jats:sup>+<\/jats:sup>\u2010free or Cl<jats:sup>\u2212<\/jats:sup>\u2010free media, suggesting that Na<jats:sup>+<\/jats:sup> and Cl<jats:sup>\u2212<\/jats:sup> were required for optimal uptake via this mechanism. These characteristics are consistent with a Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, Cl<jats:sup>\u2212<\/jats:sup> contransporter in NPE cells. Treatment of NPE cells for 15 min with phorbol 12\u2010myristate, 13\u2010acetate (PMA), an activator of protein kinase C, caused a 50\u201370% decrease in <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake via the Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, Cl<jats:sup>\u2212<\/jats:sup> cotransporter. Other <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake mechanisms were not affected. <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake via the Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, Cl<jats:sup>\u2212<\/jats:sup> contransporter could be inhibited by other phorbol esters and by dioctanoylglycerol, an analog of diacylglycerol, but not by 4\u03b1 phorbol didecanoate, an ineffective activator of protein kinase C. Staurosporine, a protein kinase C inhibitor, blocked phorbol ester inhibition of <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake. These data suggest that a Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, Cl<jats:sup>\u2212<\/jats:sup> cotransporter in NPE cells is inhibited by activation of protein kinase C. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041530126","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T07:01:26Z","timestamp":1109401286000},"page":"214-220","source":"Crossref","is-referenced-by-count":16,"title":["Potassium transport in nonpigmented epithelial cells of ocular ciliary body: Inhibition of a Na<sup>+<\/sup>, K<sup>+<\/sup>, Cl<sup>\u2212<\/sup> cotransporter by protein kinase C"],"prefix":"10.1002","volume":"153","author":[{"given":"Richard B.","family":"Crook","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas K.","family":"Von Brauchitsch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon R.","family":"Polansky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"11","article-title":"Potassium channel architecture and channel blockers","volume":"334","author":"Armstrong C. 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