{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T20:30:43Z","timestamp":1718656243258},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4478,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1992,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>When segments of rat tail artery were labeled with [<jats:sup>3<\/jats:sup>H]inositol and then stimulated with norepinephrine (NE), the inositol phosphates produced were primarily IP and IP<jats:sub>2<\/jats:sub>, together with a small but significant amount of Ins(1,4,5)P<jats:sub>3<\/jats:sub> and a very small amount of Ins(1,3,4,5)P<jats:sub>4<\/jats:sub>. It has been unclear in many studies whether or not the relatively large levels of IP and IP<jats:sub>2<\/jats:sub> produced in [<jats:sup>3<\/jats:sup>H]inositol\u2010labeled tissue represent indirect products of phosphatidyl inositol(4,5)bis phosphate breakdown (through Ins(1,4,5)P<jats:sub>3<\/jats:sub>) or direct products of phosphatidyl inositol 4 monophosphate and phosphatidyl inositol breakdown. In order to answer this question tail artery segments were prelabeled with [<jats:sup>3<\/jats:sup>H]inositol and then permeabilized with \u03b2 escin and stimulated with norepinephrine and GTP\u03b3S, so that increases in IP, IP<jats:sub>2<\/jats:sub>, and Ins(1,4,5)P<jats:sub>3<\/jats:sub> were still observed. If these permeable segments were stimulated with agonist in the presence of compounds known to inhibit Ins(1,4,5)P<jats:sub>3<\/jats:sub> 5\u2010phosphatase, such as glucose 6P, (2,3)diphosphoglycerate, or Ins(1,4,5)P<jats:sub>3<\/jats:sub>, the levels of labeled Ins(1,4,5)P<jats:sub>3<\/jats:sub> and labeled IP<jats:sub>2<\/jats:sub> were increased, while the level of stimulated labeled IP was unchanged. This indicated that some of the IP<jats:sub>2<\/jats:sub> and IP formed in these cells was produced from PIP<jats:sub>2<\/jats:sub> but that some of these compounds might be formed from PIP or PI. When the isomers of inositol monophosphate, Ins 1P and Ins 4P, were separated by HPLC, it was shown that after prelabeled tail artery was stimulated by norepinephrine for periods of 1\u20132 min, the predominant isomer formed was Ins 4P, indicating either PIP<jats:sub>2<\/jats:sub> or PIP as the source. However, after 5\u201320 min stimulation, both Ins 1P and Ins 4P were formed in equal amounts, suggesting that during sustained stimulation of smooth muscle PI itself was broken down directly. Therefore it appears that within 1\u20132 min of norepinephrine addition to vascular smooth muscle the bulk of the IP and IP<jats:sub>2<\/jats:sub> produced are derived from PIP<jats:sub>2<\/jats:sub> via IP<jats:sub>3<\/jats:sub>, while after 20 min of norepinephrine treatment much of the IP comes directly from PI. This suggests that the regulation of PLC in this tissue is more complicated than has been previously believed. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041530203","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T07:06:52Z","timestamp":1109401612000},"page":"234-243","source":"Crossref","is-referenced-by-count":5,"title":["Norepinephrine stimulates the direct breakdown of phosphatidyl inositol in rat tail artery"],"prefix":"10.1002","volume":"153","author":[{"given":"Edward F.","family":"Labelle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Snezana","family":"Trajkovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.7.3.447"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(89)90188-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1989.256.2.C375"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.001111"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/341197a0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(85)90193-1"},{"key":"e_1_2_1_8_1","first-page":"786","article-title":"Formation of inositol polyphosphates in airway smooth muscle after muscarinic receptor stimulation","volume":"252","author":"Chilvers E. R.","year":"1990","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_1_9_1","first-page":"123","article-title":"Relationship between phosphatidylinositol turnover and calcium mobilization induced by alpha\u20101 adrenoceptor stimulation in the rat aorta","volume":"240","author":"Chiu A. T.","year":"1987","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_1_10_1","first-page":"H1476","article-title":"Phosphoinositide metabolism and metabolism\u2010contraction coupling in rabbit aorta","volume":"255","author":"Coburn R. F.","year":"1988","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2410409"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"7868","DOI":"10.1016\/S0021-9258(17)39533-9","article-title":"Isolation of a phosphomonoesterase from human platelets that specifically hydrolyzes the 5\u2010phosphate of inositol 1,4,5\u2010trisphosphate","volume":"260","author":"Connolly T. M.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"16068","DOI":"10.1016\/S0021-9258(17)36201-4","article-title":"The formation of inositol 1,2\u2010cyclic phosphate on agonist stimulation of phosphoinositide breakdown in mouse pancreatic minilobules","volume":"260","author":"Dixon J. F.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2380501"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/0006-291X(85)91826-1","article-title":"Vasopressin induced production of inositol trisphosphate and calcium efflux in a smooth muscle cell line","volume":"131","author":"Doyle V. M.","year":"1985","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"e_1_2_1_16_1","first-page":"54","article-title":"Polyphosphoinositides are the major source of inositol phosphates in carbamoylcholine\u2010stimulated SK\u2010N\u2010SH neuroblastoma cells","volume":"38","author":"Fisher S. K.","year":"1990","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(85)90195-5"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(87)90751-5"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)91017-5"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/288373a0"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"5901","DOI":"10.1016\/S0021-9258(17)38468-5","article-title":"Sustained diacylglycerol formation from inositol phosphates in angiotensin II stimulated vascular smooth muscle cells","volume":"261","author":"Griendling K. K.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1991.261.1.C17"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)75741-X"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2520583"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp015953"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2430457"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"9400","DOI":"10.1016\/S0021-9258(18)60545-9","article-title":"Source of 3H\u2010labeled inositol bis\u2010 and monophosphates in agonist\u2010activated rat parotid acinar cells","volume":"264","author":"Hughes A. R.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.8.2170"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.11.6.627"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(89)90156-2"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017267"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","first-page":"17997","DOI":"10.1016\/S0021-9258(19)84670-7","article-title":"Cytosolic heparin inhibits muscarinic and \u03b1\u2010adrenergic Ca2+ release in smooth muscle","volume":"264","author":"Kobayashi S.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041440305"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80980-W"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)80922-2"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(85)90571-0"},{"key":"e_1_2_1_37_1","doi-asserted-by":"crossref","first-page":"3051","DOI":"10.1016\/S0021-9258(18)69030-1","article-title":"Inositol phosphates: Synthesis and degradation","volume":"263","author":"Majerus P. W.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(86)80515-0"},{"key":"e_1_2_1_39_1","doi-asserted-by":"crossref","first-page":"13979","DOI":"10.1016\/S0021-9258(18)68171-2","article-title":"Biochemical events associated with activation of smooth muscle contraction","volume":"263","author":"Miller\u2010Hance W. C.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_40_1","doi-asserted-by":"crossref","first-page":"5658","DOI":"10.1016\/S0021-9258(19)67645-3","article-title":"Potassium channels regulated by Inositol 1,3,4,5\u2010tetrakisphosphate and internal calcium in DDT1 MF\u20102 smooth muscle cells","volume":"266","author":"Molleman A.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89121-9"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)80303-6"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)80831-3"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.258.1.C122"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2450305"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2540553"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2390567"},{"key":"e_1_2_1_48_1","doi-asserted-by":"crossref","first-page":"13791","DOI":"10.1016\/S0021-9258(18)68312-7","article-title":"Platelet\u2010activating factor\u2010induced homologous and heterologous desensitization in cultured vascular smooth muscle cells","volume":"263","author":"Schwertschlag U. S.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_49_1","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/S0021-9258(19)75932-8","article-title":"The formation of inositol 1,2\u2010cyclic 4,5\u2010trisphosphate and inositol 1,2\u2010cyclic 4\u2010bisphosphate on stimulation of mouse pancreatic minilobules with carbamylcholine","volume":"262","author":"Sekar M. C.","year":"1987","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.15.5231"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)91279-8"},{"key":"e_1_2_1_52_1","first-page":"H1211","article-title":"Endothelium\u2010dependent dilation to L\u2010arginine in isolated rat skeletal muscle arterioles","volume":"262","author":"Sun D.","year":"1992","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/327249a0"},{"key":"e_1_2_1_54_1","doi-asserted-by":"crossref","first-page":"11718","DOI":"10.1016\/S0021-9258(20)71269-X","article-title":"Hydrolysis of polyphosphoinositides by purified sheep seminal vesicle phospholipase C enzymes","volume":"259","author":"Wilson D. B.","year":"1984","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2551015"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb19248.x"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2510129"},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90412-7"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041530203","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041530203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T13:28:49Z","timestamp":1698154129000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041530203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,11]]}},"alternative-id":["10.1002\/jcp.1041530203"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041530203","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11]]}}}