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Whereas 0.01 nM and 0.1 nM bFGF\u2010SAP stimulate BEL cells proliferation, 1 nM and 10 nM bFGF\u2010SAP have the predicted toxic effects on BEL cell growth. The toxicity of bFGF\u2010SAP is observed 2 to 3 days after the initial treatment and depends on cell density. Accordingly, the sensitivity of confluent cells to bFGF\u2010SAP is reduced compared to sparse cells. A time course analysis reveals that bFGF\u2010SAP is effective after a short exposure to cells and that its effects are not increased with longer treatments. Cell growth on bFGF\u2010SAP pretreated extracellular matrix (ECM) or posterior lens capsule (PLC) is also affected. Basic FGF\u2010SAP has been shown to bind to the extracellular material, allowing a modulation of lens cells migration and survival by a single treatment in vitro. This finding raises the possibility of its use in vivo to prevent capsules invasion by lens cells after cataract surgery. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041530307","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T07:11:04Z","timestamp":1109401864000},"page":"483-490","source":"Crossref","is-referenced-by-count":9,"title":["Biphasic effect of the mitotoxin bFGF\u2010saporin on bovine lens epithelial cell growth: Effect of cell density and extracellular matrix"],"prefix":"10.1002","volume":"153","author":[{"given":"Thierry","family":"David","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Tassin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas A.","family":"Lappi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Baird","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yves","family":"Courtois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(78)90142-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a072333"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00348a001"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90183-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(89)80120-2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.17.5364"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(85)80056-0"},{"key":"e_1_2_1_9_1","first-page":"393","article-title":"A heparin\u2010binding angiogenic protein basic fibroblast growth factor is stored within basement membrane","volume":"130","author":"Folkman J.","year":"1988","journal-title":"J. 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