{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:02:48Z","timestamp":1760644968574},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4386,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Addition of polyamines or their analogs to newly confluent LLC\u2010PK<jats:sub>1<\/jats:sub> cells resulted in down\u2010regulation of Na<jats:sup>+<\/jats:sup>\u2010glucose transport (symport) activity. Polyamines prevented the induction of this symporter by the differentiation inducer hexamethylene bisacetamide (HMBA) but did not influence induction by the phosphodiesterase inhibitor 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX). Partial depletion of endogenous polyamines after addition of \u03b1 \u2010difluoromethylornithine (DFMO) resulted in a 4 to 5\u2010fold increase in symporter expression. Symporter induction by either HMBA or DFMO was inhibited by the protein kinase inhibitor H\u20107 but H\u20107 did not affect symporter induction by IBMX. Changes in symporter activity were accompanied by changes in levels of the 75 kD symporter subunit detected by Western blot. Cultures exposed to HMBA exhibited reduced levels of ornithine decarboxylase activity. Our results suggest that induction of symporter expression by HMBA may be mediated in part by its effects on polymine metabolism, and point to parallel roles of polyamines and cyclic AMP in regulating the expression of this physiologically important renal transport system. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041540205","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:40:42Z","timestamp":1109400042000},"page":"238-247","source":"Crossref","is-referenced-by-count":15,"title":["Polyamine regulation of Na<sup>+<\/sup>\/glucose symporter expression in LLC\u2010PK<sub>1<\/sub> cells"],"prefix":"10.1002","volume":"154","author":[{"given":"Hua","family":"Peng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia E.","family":"Lever","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2390363"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.242.1.C94"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041220214"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1991.260.6.C1290"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2690035"},{"key":"e_1_2_1_7_1","first-page":"4900","article-title":"Identification of metabolites of the cell\u2010differentiating agent hexamethylene bisacetamide in humans","volume":"46","author":"Callery P. 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