{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T12:48:47Z","timestamp":1783774127122,"version":"3.55.0"},"reference-count":65,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4386,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Progressive stenosis or occlusion of bilateral internal carotid arteries by fibrocellular intimal thickening results in cerebral ischemia in moyamoya disease. We recently found that cultured smooth muscle cells (SMC) derived from arteries of patients with moyamoya disease responded poorly to serum mitogens, especially to platelet\u2010derived growth factor (PDGF). In the present study, we investigated further the binding and processing of <jats:sup>125<\/jats:sup>I\u2010PDGF, as well as down\u2010regulation of the PDGF receptors in arterial SMC derived from patients with moyamoya disease. The specific binding sites of <jats:sup>125<\/jats:sup>I\u2010PDGF were reduced significantly at both 4\u00b0C and 22\u00b0C on SMC from moyamoya disease compared with those from controls (4.78 vs. 11.92 \u00d7 10<jats:sup>4<\/jats:sup>\/cell at 4\u00b0C), though the apparen dissociation constant (Kd) were the same. Kinetics of <jats:sup>125<\/jats:sup>I\u2010PDGF binding at 37\u00b0C in cells from moyamoya disease showed fewer binding sites (less than 1\/3 of controls) and lower degradation per cell than in those from controls, though no difference was observed in either internalization or degradation of each receptor. When SMC were exposed to lower concentrations of nonlabeled PDGF at 37\u00b0C, the percentage of remaining binding sites on cells from moyamoya disease was significantly less than that from controls. This excess down\u2010regulation of PDGF receptor in SMC from moyamoya disease may be interpreted as insufficent recycling or a decreased intracellular pool of the PDGF receptor. These results are closely correlated with the diminished proliferation responses to PDGF in SMC from moyamoya disease and provide evidence that functional alterations in vascular cells are involved in the mechanism of development of intimal thickening in moyamoya disease. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041540210","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:40:42Z","timestamp":1109400042000},"page":"281-288","source":"Crossref","is-referenced-by-count":32,"title":["Kinetics of <sup>125<\/sup>I\u2010PDGF binding and down\u2010regulation of PDGF receptor in arterial smooth muscle cells derived from patients with moyamoya disease"],"prefix":"10.1002","volume":"154","author":[{"given":"Masaru","family":"Aoyagi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Fukai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiharu","family":"Matsushima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyotaka","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041470202"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3022382"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"11699","DOI":"10.1016\/S0021-9258(18)80121-1","article-title":"Ligan\u2010induced dimerization of the platelet\u2010derived growth factor receptor Monomer\u2010dimer interconversion occurs independent of receptor phosphorylation","volume":"264","author":"Bishayee S.","year":"1989","journal-title":"J. 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