{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T20:36:07Z","timestamp":1718656567042},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4358,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1993,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The relative contributions of Na<jats:sup>+<\/jats:sup>\u2010<jats:sup>+<\/jats:sup>\u2010pumps and Na<jats:sup>+<\/jats:sup>\u2010<jats:sup>+<\/jats:sup>\u2010<jats:sup>\u2212<\/jats:sup> contransport to total rubidium (Rb<jats:sup>+<\/jats:sup>) influx into primary cultures of renal tubule cells (PC.RC) and cells transformed either with the wild\u2010type or a temperature\u2010sensitive mutant of the simian virus 40 (SV40), were measured under various growth conditions. The Na<jats:sup>+<\/jats:sup>\u2010<jats:sup>+<\/jats:sup>\u2010mediated component represented 74% and 44\u201348% of total Rb<jats:sup>+<\/jats:sup> influx into PC.RC and SV40\u2010transformed cells, respectively. Proliferating transformed cells showed substantial ouabain\u2010resistant bumetanide\u2010sensitive (Or\u2010Bs) Rb<jats:sup>+<\/jats:sup> influx (41\u201345% of total) which indicated the presence of Na<jats:sup>+<\/jats:sup>\u2010<jats:sup>+<\/jats:sup>\u2010<jats:sup>\u2212<\/jats:sup> contransport. The Or\u2010Bs component of Rb<jats:sup>+<\/jats:sup> influx was greatly reduced when temperature\u2010sensitive transformed renal cells (RC.SV<jats:sub>ts<\/jats:sub>A<jats:sub>58<\/jats:sub>) grown in Petri dishes or on permeable filters were shifted from the permissive (33\u00b0C) to the restrictive temperature (39.5\u00b0C) to arrest cell growth. The ouabain\u2010sensitive Rb<jats:sup>+<\/jats:sup> influx mediated by the Na<jats:sup>+<\/jats:sup>\u2010<jats:sup>+<\/jats:sup>\u2010, the total and amiloride\u2010sensitive Na<jats:sup>+<\/jats:sup> uptakes were not modified following inhibition of cell proliferation. A similar fall in the Or\u2010Bs influx was obtained when renal tubule cells transformed by the wild\u2010type SV40 (RC.SV) were incubated with the K<jats:sup>+<\/jats:sup> channel blocker, tetraethylammonium (TEA) ion, which we had previously shown to arrest cell growth without affecting cell viability (Teulon et al.: J. Cell. Physiol., 151:113\u2013125, 1992). Reinitiation of cell growth by removal of TEA or return to 33\u00b0C of the temperature\u2010sensitive cells restored the Or\u2010Bs component of Rb influx. Taken together, these results indicate that the Na<jats:sup>+<\/jats:sup>\u2010<jats:sup>+<\/jats:sup>\u2010<jats:sup>\u2212<\/jats:sup> cotransport activity is critically dependent on cell growth con ditions. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041540304","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:45:20Z","timestamp":1109400320000},"page":"466-477","source":"Crossref","is-referenced-by-count":9,"title":["K<sup>+<\/sup> fluxes mediated by Na<sup>+<\/sup>\u2010K<sup>+<\/sup>\u2010Cl<sup>\u2212<\/sup> cotransport and Na<sup>+<\/sup>\u2010K<sup>+<\/sup>\u2010ATPase pumps in renal tubule cell lines transformed by wild\u2010type and temperature\u2010sensitive strains of simian virus 40"],"prefix":"10.1002","volume":"154","author":[{"given":"A.","family":"Vandewalle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Vuillemin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Teulon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Baudouin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Wahbe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Cassing\u00e9na","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Ronco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041230216"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041320302"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.96.2.330"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041130107"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0710465"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.3.1409"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(85)09104-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/307465a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1989.69.2.417"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.85.3.651"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041350210"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041410126"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01872124"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(77)90120-3"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.20.6275"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(80)91483-7"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/304645a0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1987.253.2.C177"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1016\/S0021-9258(18)89612-0","article-title":"Na\/K\/Cl cotransport in cultured human fibroblasts","volume":"260","author":"Owens N. 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