{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:09:45Z","timestamp":1779340185302,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4266,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1993,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two complementary experimental methods have been used to examine mitogen\u2010induced transmembrane conductances in human B cells using the Daudi cell line as a model for human B cell activation. Spectrofluorometry was used to investigate mitogen\u2010induced changes in [Ca<jats:sup>++<\/jats:sup>]<jats:sub>i<\/jats:sub> and transmembrane potential. Activation of human B cells with anti\u2010\u03bc antibodies resulted in a biphasic rise in [Ca<jats:sup>++<\/jats:sup>]<jats:sub>i<\/jats:sub>, the second phase being mediated by the influx of extracellular Ca<jats:sup>++<\/jats:sup>. Ca<jats:sup>++<\/jats:sup> influx was inhibited by high [K<jats:sup>+<\/jats:sup>]e, suggesting that this influx was transmembrane potential sensitive. Membrane currents of Daudi cells were investigated using voltage clamp techniques. Before mitogenic stimulation, the cells were electrically quiet. Within several minutes of the addition of anti\u2010\u03bc antibodies to the bath solution, inward currents were observed at negative voltages. Whole\u2010cell currents changed instantly with voltage steps and were transmembrane potential sensitive in that at potentials more positive than \u221240 mV no currents were detectable. A similar conductance was also activated by the introduction of IP<jats:sub>3<\/jats:sub> into the intracellular solution, suggesting that IP<jats:sub>3<\/jats:sub> generation after surface IgM crosslinking is involved in the activation of this conductance. Both anti\u2010\u03bc and IP<jats:sup>3<\/jats:sup> induced currents were blocked by 1 mM La<jats:sup>+++<\/jats:sup>, which is known to block Ca<jats:sup>++<\/jats:sup> channels. These results strongly support the presence of membrane Ca<jats:sup>++<\/jats:sup> channels in human B cells that function in the early stages of activation. Changes in transmembrane potential appear to be important in regulating Ca<jats:sup>++<\/jats:sup> influx. These mechanisms work in concert to regulate the level of [Ca<jats:sup>++<\/jats:sup>]<jats:sub>i<\/jats:sub> during the early phases of human B cell activation. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041550310","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:27:40Z","timestamp":1109399260000},"page":"520-529","source":"Crossref","is-referenced-by-count":12,"title":["Mitogen\u2010activated Ca<sup>++<\/sup> channels in human B lymphocytes"],"prefix":"10.1002","volume":"155","author":[{"given":"Lawrence H.","family":"Brent","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qihua","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven J.","family":"Wieland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2200345"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80080-5"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91238-6"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"15644","DOI":"10.1016\/S0021-9258(18)66764-X","article-title":"Quantitative changes in inositol 1,4,5\u2010trisphosphate in chemoattractant\u2010stimulated neutrophils","volume":"261","author":"Bradford P. 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