{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T03:46:34Z","timestamp":1752551194081},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4236,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1993,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One major approach to the study of growth factor receptor action has been to overexpress wild\u2010type or mutant receptors in cultured cells and to evaluate biological responses to exogenous ligand. Studies of this type with insulin and insulin\u2010like growth factor\u2010I (IGF\u2010I) receptors often use Chinese hamster ovary (CHO) cells. We have compared the effect of receptor overexpression in CHO cells and in NIH\u20103T3 fibroblasts in order to assess the suitability of CHO cells for studies of this nature and the contribution of cell type\u2010specific factors to those responses generally assayed. Overexpression of IGF\u2010I receptors in NIH\u20103T3 cells resulted in increased sensitivity and maximal responsiveness of thymidine incorporation, 2\u2010deoxyglucose uptake, and phosphatidylinositol\u20103 (PI3) kinase activation to IGF\u2010I stimulation. In CHO cells, on the other hand, overexpression of either IGF\u2010I or insulin receptors increased the sensitivity of thymidine incorporation to ligand, but maximal responsiveness was unchanged or decreased. Overexpression of the insulin receptor increased sensitivity of glucose uptake and the maximal response of PI3 kinase activation to insulin. Overexpression of the IGF\u2010I receptor did not affect sensitivity or maximal responsiveness of glucose uptake or PI3 kinase activation to IGF\u2010I. These data suggest that IGF\u2010I and insulin signal pathways may differ in CHO cells, and that there may even be divergent IGF\u2010I signaling pathways for short vs. long\u2010term effects. Whether this is a result of differences in the number of endogenous receptors, hybrid receptor formation, or defects in post\u2010receptor signaling, the use of CHO cells to assess receptor function must be approached with caution. \u00a9 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041560120","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T06:28:13Z","timestamp":1109399293000},"page":"145-152","source":"Crossref","is-referenced-by-count":15,"title":["Paradoxical biological effects of overexpressed insulin\u2010like growth factor\u20101 receptors in chinese hamster ovary cells"],"prefix":"10.1002","volume":"156","author":[{"given":"Hisanori","family":"Kato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa N.","family":"Faria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bethel","family":"Stannard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel","family":"Levy\u2010Toledano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simeon I.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Charles T.","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek","family":"Leroith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"151","article-title":"How distinct are the insulin and insulin\u2010like growth factor I signalling systems?","volume":"3","author":"Adamo M.","year":"1991","journal-title":"Bio\u2010Factors"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"12788","DOI":"10.1016\/S0021-9258(18)42345-9","article-title":"Enhanced insulin\u2010induced mitogenesis and mitogen\u2010activated protein kinase activities in mutant insulin receptors with substitution of two COOH\u2010terminal tyrosine autophosphorylation sites by phenylalanine","volume":"267","author":"Ando A.","year":"1992","journal-title":"J. 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