{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T07:32:43Z","timestamp":1779262363274,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4052,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we examined the role of the bumetanide\u2010sensitive Na+\/K+\/Cl\u2013cotransport in the mitogenic signal of vascular endothelial cell proliferation. The activity of the Na+\/K+\/Cl\u2013 cotransport is dramatically decreased in quiescent subconfluent cells, as compared to subconfluent cells growing in the presence of FGF. The Na+\/K+\/Cl\u2013 cotransport activity of quiescent subconfluent cultures deprived of FGF decreased to 6%, whereas that of quiescent cells grown to confluency was reduced to only 33% of the activity of subconfluent cells growing in the presence of FGF. The basal low activity of Na+\/K+\/Cl\u2013 cotransport in the quiescent subconfluent vascular endothelial cells was dramatically stimulated by FGF. In order to explore the role of the Na+\/K+\/Cl\u2013 cotransport in the mitogenic signal of the endothelial cells, the effect of two specific inhibitors of the cotransport \u2010furosemide and \u2010bumetanide was tested on cell proliferation induced by FGF. Bumetanide and furosemide inhibited synchronized cell proliferation measured by direct counting of cells and by DNA synthesis. Inhibition by fuorsemide and bumetanide was reversible; removal of these compounds completely released the cells to proliferate. These results indicate that the effect of these drugs is specific and is not due to an indirect toxic effect. This study clearly demonstrates that the FGF\u2010induced activation of the Na+\/K+\/Cl\u2013 cotransport plays a role in the mitogenic signal pathway of vascular endothelial cells. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041580115","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T05:02:30Z","timestamp":1109394150000},"page":"121-127","source":"Crossref","is-referenced-by-count":47,"title":["Bumetanide and furosemide inhibited vascular endothelial cell proliferation"],"prefix":"10.1002","volume":"158","author":[{"given":"Rivka","family":"Panet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Markus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henri","family":"Atlan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(87)90586-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041230216"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01868712"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1986.250.6.C888"},{"key":"e_1_2_1_6_1","first-page":"721","volume-title":"Pharmacological basis of therapeutics","author":"Goodman","year":"1990"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040910307"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"20326","DOI":"10.1016\/S0021-9258(19)47066-X","article-title":"[3H]Bumetanide binding in vascular endothelial cells. 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