{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T22:03:10Z","timestamp":1758405790819},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3871,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1994,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epoxyeicosatrienoic acids (EETs) are arachidonic acid metabolites formed endogenously via the cytochrome P450 pathway in rat, rabbit, and human kidney. We characterized the effects of the four regioisomeric EETs on ion transport in the renal epithelial cell line, LLC\u2010PK<jats:sub>1<\/jats:sub>. Among the EETs, 14, 15\u2010EET was the most potent inhibitor of <jats:sup>86<\/jats:sup>Rb uptake. Its effect was concentration\u2010dependent (IC<jats:sub>50<\/jats:sub> = 75 nM) and stereoselective to the 14S, 15R\u2010EET. Experiments measuring 14, 15\u2010EET\u2013induced <jats:sup>86<\/jats:sup>Rb uptake inhibition in the presence of inhibitors of Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2010ATPase activity (ouabain), Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2010Cl<jats:sup>\u2212<\/jats:sup> cotransporter (furosemide), and Na<jats:sup>+<\/jats:sup>\u2010H<jats:sup>+<\/jats:sup> exchanger (amiloride) suggested that 14, 15\u2010EET inhibits ion transport via an amiloride\u2010sensitive mechanism. These results, together with previous reports demonstrating their endogenous production in the kidney, suggest an important role for EETs, specifically 14, 15\u2010EET, in the regulation of ion and water reabsorption in the kidney and implicate their function in renal pathophysiology. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041600109","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T04:45:12Z","timestamp":1109393112000},"page":"69-74","source":"Crossref","is-referenced-by-count":12,"title":["Effects of epoxyeicosatrienoic acids on <sup>86<\/sup>Rb uptake in renal epithelial cells"],"prefix":"10.1002","volume":"160","author":[{"given":"R.","family":"Staudinger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. G.","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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