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To provide a basis for studies at the molecular level, a directional expression cDNA library was constructed in Uni\u2010ZAP XR vector from poly A<jats:sup>+<\/jats:sup> RNA of the human non\u2010pigmented ciliary epithelial derived ODM\u20102 cell line. Fifty\u2010three cDNA clones were isolated from the library and characterized by partial sequence analysis. Approximately 49% of the clones exhibited homology with known genes in the GenBank\/EMBL databases. The putative identification of these clones may reflect the transcriptional activity of the ODM\u20102 cells in culture. One of the identified clones, ODM\u201042\u2010I, was found to be specific and highly expressed in the corneal epithelium. This clone had an exact match with a recently discovered human gene, \u03b2ig\u2010h3 (Skonier et al., 1992, DNA Cell Biol., <jats:italic>11<\/jats:italic>: 511\u2013522), which codes a surface recognition protein, inducible by transforming growth factor beta (TGF\u2010\u03b2), and containing a putative binding site (RDG) for integrins. The ODM\u201042\u2010I cDNA clone displays a distinctive pattern of expression found in the human eye, expressed almost exclusively in the cornea. Further studies, using sera from a synthetic peptide to the carboxy\u2010terminal region of ODM\u201042\u2010I, reveal that the protein is heterogeneous in charge and is preferenctially expressed on the extracellular surface of corneal epithelial cells, and might share immunologic properties with integrins \u03b21. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041600314","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T04:53:45Z","timestamp":1109393625000},"page":"511-521","source":"Crossref","is-referenced-by-count":93,"title":["cDNA from human ocular ciliary epithelium homologous to \u03b2ig\u2010h3 is preferentially expressed as an extracellular protein in the corneal epithelium"],"prefix":"10.1002","volume":"160","author":[{"given":"Julio","family":"Escribano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nati","family":"Hernando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sikha","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Coca\u2010Prados","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Crabb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/355632a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0793-256"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713689109013867"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(05)80207-6"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.22.8754"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713689209000061"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.45.3.202"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"16674","DOI":"10.1016\/S0021-9258(18)55354-0","article-title":"Topological and epitope mapping of the cellular retinaldehyde\u2010binding protein from retina","volume":"266","author":"Crabb J. 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