{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T15:51:37Z","timestamp":1772812297542,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3779,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exposure of non\u2010excitatory cells to the tyrosine kinase (PTK) inhibitors, genistein, herbimycin A, and tyrphostin, induced at least two families of K<jats:sup>+<\/jats:sup> currents. The first, a TEA\u2010insensitive slow\u2010inactivating K<jats:sup>+<\/jats:sup> current, is induced within 3 min following treatment with 140 mM genistein or 100 nM herbimycin A. The second current, a TEA\u2010sensitive delayed rectifier, is induced within 30 min following treatment with 50 mM genistein or 10 nM herbimycin A. Currents with similar biophysical and pharmacological characteristics are induced in these cells following exposure to ionizing radiation. The radiation\u2010induced currents are inhibited by pretreatment with the free radical scavenger, N\u2010Acetyl L\u2010Cysteine, or by pretreatment with the protein kinase C inhibitor, staurosporine; those induced by PTK inhibitors are not. The latter, therefore, do not appear to be mediated through free radicals or require serine\/threonine phosphorylation for activation. Once the channels are activated by the PTK inhibitors, phosphorylation of the channel at serine\/threonine residues results in slower inactivation of the induced current. We propose that protein tyrosine phosphorylation of the K<jats:sup>+<\/jats:sup> channel protein itself or of a factor that interacts with it maintains the K<jats:sup>+<\/jats:sup> channels of non\u2010excitatory cells in a closed state. Following exposure to ionizing radiation, free radical\u2010induced activation of serine\/threonine kinase(s) results in phosphorylation of the channel and\/or inactivation of a tyrosine kinase that in turn leads to activation of the K<jats:sup>+<\/jats:sup> channels. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041610117","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T04:54:21Z","timestamp":1109393661000},"page":"142-148","source":"Crossref","is-referenced-by-count":7,"title":["Modulation of potassium channels by protein tyrosine kinase inhibitors"],"prefix":"10.1002","volume":"161","author":[{"given":"A. H.","family":"Saad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. S.","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. C.","family":"Koong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. M.","family":"Hahn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Giaccia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.63.5.533"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)81364-6"},{"key":"e_1_2_1_4_1","volume-title":"Ionic Channels of Excitable Membranes","author":"Hille B.","year":"1992"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1242\/jeb.124.1.375","article-title":"Phorbol esters, protein phosphorylation and the regulation of neuronal ion channels","volume":"124","author":"Kaczmarek L. K.","year":"1986","journal-title":"J. Exp. Biol."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(87)90122-6"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1708916"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041560217"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.3.908"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.11.030188.001003"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.12.2.609"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.5227"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90504-6"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01945518"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(87)90145-3"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80579-6"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.20.9396"},{"issue":"4","key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.4049\/jimmunol.151.4.1918","article-title":"Anti\u2010TAPA\u20101 antibodies induce protein tyrosine phosphorylation that is prevented by increasing intracellular thiol levels","volume":"151","author":"Schick M. R.","year":"1993","journal-title":"J. Immunol."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)80119-N"},{"key":"e_1_2_1_20_1","first-page":"211","article-title":"Modulation of ion channal activity: A key funcion of the protein kinase C enzyme family","volume":"41","author":"Shearman M. S.","year":"1989","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb08483.x"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041610117","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041610117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T06:46:31Z","timestamp":1698216391000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041610117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1002\/jcp.1041610117"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041610117","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}