{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:21:31Z","timestamp":1770747691559,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3748,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1994,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We tested the hypothesis that mineralocorticoids potentiate angiotensin II\u2013stimulated phospholipase C activation through an increased number of angiotensin II receptors in cultured rat aortic vascular smooth muscle cells. Exposure of cells to aldosterone for 24 h resulted in concentration\u2010dependent increases in angiotensin II receptor binding. Via studies of angiotensin II displacement by non\u2010peptide receptor antagonists, both basal and upregulated angiotensin II receptors were found to be of the AT<jats:sub>1<\/jats:sub>, subtype. Incubation with 1 \u03bcM aldosterone resulted in 50%\u2013100% enhancement of angiotensin II (100 nM)\u2013stimulated diacylglycerol formation and intracellular calcium mobilization. Exposure to 100 nM 1,25\u2010(OH)<jats:sub>2<\/jats:sub> VitD<jats:sub>3<\/jats:sub>, which did not upregulate angiotensin II receptors, did not potentiate stimulated inositol phosphate formation. Incubation with aldosterone resulted in potentiation of inositol phosphate formation upon receptor occupation (100 nM angiotensin II) but not upon post\u2010receptor stimulation (25 mM NaF\/10 \u03bcM AlCl<jats:sub>3<\/jats:sub>). Aldosterone did not increase basal phospholipase C activity or content of the inositol trisphosphate precursor phosphatidylinositol\u20104,5\u2010bisphosphate. These data are consistent with the hypothesis that aldosterone potentiates angiotensin II\u2013stimulated, phospholipase C\u2010dependent intracellular signals solely by coupling to an increased number of angiotensin II receptors. This mechanism may contribute to the sensitized vascular responses to angiotensin II observed in states of mineralocorticoid excess. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041610203","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T05:01:45Z","timestamp":1109394105000},"page":"201-208","source":"Crossref","is-referenced-by-count":27,"title":["Mechanisms of enhanced angiotensin II\u2013stimulated signal transduction in vascular smooth muscle by aldosterone"],"prefix":"10.1002","volume":"161","author":[{"given":"Michael E.","family":"Ullian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana J.","family":"Fine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.42.6.764"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.20.3.304"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.20.1.59"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/10641968009038550"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2845584"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-125-4-2224"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.9.6_Pt_2.III181"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.87.6.1816"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicator with greatly improved fluorescence properties","volume":"260","author":"Grynkiewicz G.","year":"1985","journal-title":"J. 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