{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:39:13Z","timestamp":1761154753017},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3656,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1995,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human omental microvascular endothelial (HOME) cells seeded on Matrigel begin to migrate within 1 h, forming honeycomb\u2010like structures and capillary\u2010like networks within 18 h. Cross\u2010sections of the capillary networks show them to be tube\u2010like structures. Northern blot analysis showed that tissue\u2010type plasminogen activator (t\u2010PA) mRNA synthesis increased from the initial state at 0 h after seeding on Matrigel, reaching a steady state after 4 h. This elevated cellular t\u2010PA mRNA level decreased markedly at 24 h. In contrast, the cellular plasminogen activator inhibitor\u20101 (PAI\u20101) mRNA level demonstrated biphasic curves during the 24 h after seeding on Matrigel: the PAI\u20101 mRNA level was increased eightfold initially at 4 h over that at O h, then declined, and again secondarily increased to greater than tenfold at 18 h. Cellular levels of both 72 kD type IV collagenase and tissue inhibitor of metalloproteinase (TIMP\u20102) mRNA were increased only a slightly within 2\u20134 h. These elevated mRNA levels were maintained for 18 h, while the TIMP\u20101 mRNA level increased up to 18 h, reaching around three times the level at O h. However, on collagen\u2010coated dishes, cellular levels of t\u2010PA, PAI\u20101, 72 kD type IV collagenase, TIMP\u20101, and TIMP\u20102 mRNA were not greatly changed during incubation for 24 h. On Matrigel, the cellular t\u2010PA mRNA level at 18 h after seeding was greatly increased when treated with specific anti\u2010transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) antibody. In contrast, both PAI\u20101 and TIMP\u20101 mRNA levels at 18 h were reduced in the presence of anti\u2010TGF\u2010\u03b2 antibody. Development of the capillary network on Matrigel was inhibited in the presence of anti\u2010t\u2010PA antibody. Epidermal growth factor (EGF) enhanced t\u2010PA gene expression and TGF\u2010\u03b2 inhibited its expression in HOME cells cultured on collagen\u2010coated dishes. On the other hand, TGF\u2010\u03b2 enhanced cellular expression of the PAI\u20101 gene. The formation of a capillary network by HOME cells on Matrigel appears to be balanced by angiogenic EGF and anti\u2010angiogenic TGF\u2010\u03b2 through modulation of PA activity. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041620207","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T04:03:56Z","timestamp":1109390636000},"page":"213-224","source":"Crossref","is-referenced-by-count":22,"title":["Expression of tissue\u2010type plasminogen activator and its inhibitor couples with development of capillary network by human microvascular endothelial cells on matrigel"],"prefix":"10.1002","volume":"162","author":[{"given":"Ken\u2010Ichi","family":"Ito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Ryuto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin","family":"Ushiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Ono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Sugenoya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akio","family":"Kuraoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yosaburo","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michihiko","family":"Kuwano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041530302"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.8.2407"},{"key":"e_1_2_1_4_1","first-page":"701","article-title":"Inhibition of metalloproteinase, metastasis in cells transfected with an inhibitor of metalloproteinases","volume":"52","author":"Declerck Y. 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