{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T19:08:34Z","timestamp":1751483314877},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3475,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Membrane current and [Ca]<jats:sub>i<\/jats:sub> in rabbit nonpigmented ciliary body epithelial cells (NPE cells) were monitored with combined patch\u2010clamp and fura\u20102 measurements during cell swelling induced by anisosmotic conditions. In the presence of K\u2010channel blockers, cell swelling produced an increase in membrane current, accompanied by an increase in [Ca]<jats:sub>i.<\/jats:sub> Structural changes in the cell, associated with membrane deformation, may be the cause of the increase in [Ca]<jats:sub>i<\/jats:sub> during swelling. The conductance activated by swelling was permeable to CI: it was dependent on the CI concentration gradient across the cell membrane, and it was blocked by the CI\u2010channel blockers DIDS, SITS, NPPB, and DIOA. Although swelling increased both CI current and [Ca]<jats:sub>i<\/jats:sub>, there was no evidence that Ca was involved in the regulation of the CI conductance. Cell swelling activated the current even when [Ca]<jats:sub>i<\/jats:sub> was strongly buffered at an elevated level (500 nM) or at a low level (\u223c0) with internal Ca\u2010BAPTA\/Cs\u2010BAPTA mixtures. In addition, CI conductance was unaffected when [Ca]<jats:sub>i<\/jats:sub> was increased with a Ca ionophore. There was also no evidence that cAMP participates in the regulation of the CI conductance: swelling activation of the current occurred in the presence of cAMP inhibitor (R<jats:sub>p<\/jats:sub>\u2010cAMP\u2010S) and cAMP mimic (S<jats:sub>p<\/jats:sub>\u2010cAMP\u2010S). The data suggest independent involvement of CI conductance and internal Ca in the regulation of cell volume in NPE cells. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041640209","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T04:33:51Z","timestamp":1109392431000},"page":"286-294","source":"Crossref","is-referenced-by-count":12,"title":["Swelling activates chloride current and increases internal calcium in nonpigmented epithelial cells from the rabbit ciliary body"],"prefix":"10.1002","volume":"164","author":[{"given":"Lev M.","family":"Botchkin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","DOI":"10.1152\/ajpcell.1993.265.4.C1037","article-title":"Chloride current activated by swelling in retinal pigment epithelium cells","volume":"265","author":"Botchkin L. 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