{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T15:11:15Z","timestamp":1698419475382},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3444,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have investigated the phenotypic and functional characteristics of murine pre\u2010B cells obtained in semisolid and liquid culture with stem cell factor (SCF) and interleukin 7 (IL\u20107). Both serum\u2010supplemented and serum\u2010deprived culture conditions were used. The source of bone marrow cells was either normal mice (CD1 and C3H) or the lupus strain of mice MRL\/Ipr and its congenic strain MRL\/+. SCF (100 ng\/ml) and IL\u20107 (250 ng\/ml) supported murine B cell proliferation in vitro from all the murine strains analyzed both in serum\u2010supplemented and serum\u2010deprived conditions. Maximal colony growth was observed in both cases when the factors were used in combination. The growth factors alone induced some colony growth in serum\u2010supplemented cultures but were either ineffective or had modest activity in serum\u2010deprived cultures. Cells harvested from the colonies or generated in liquid cultures and stimulated with SCF + IL\u20107 in the absence of serum had almost exclusively a pre\u2010B cell phenotype (BP\u20101+, B220+, slg\u2010, CD4\u2010, CD8\u2010, Mac\u20101, RB\u20106\u2010). Both the maximal colony growth in semisolid culture and the maximal number of cells in liquid culture were observed at day 12\u201314. At this time, the pre\u2010B cells failed to differentiate further and started to die. Pre\u2010B cells generated in vitro were, however, capable of differentiating in vivo. SCID mice injected with 2 \u00d7 10<jats:sup>6<\/jats:sup> pre\u2010B cells had readily detectable serum levels of IgM (54 \u00b1 26 m\u0308g\/ml) and IgG (60 \u00b1 95 m\u0308g\/ml) at 4 weeks and 6 weeks posttransplantation, respectively. Mature B and T cells of the donor major histocompatibility complex type were detected in the SCID mice at sacrifice 14 weeks posttransplantation. These data indicate that purified (&gt;80% BP\u20101+) populations of functional pre\u2010B cells can be grown from murine bone marrow of normal mice as well as of lupus mice in serum\u2010deprived cultures stimulated with SCF and IL\u20107. These cultures, therefore, provide a highly enriched source of pre\u2010B cells but also contain T cell precursors that differentiate upon adoptive transfer into SCID mice. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041640314","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T02:06:44Z","timestamp":1109383604000},"page":"562-570","source":"Crossref","is-referenced-by-count":5,"title":["Functional characterization of lymphoid cells generated in serum\u2010deprived culture stimulated with stem cell factor and interleukin 7 from normal and autoimmune mice"],"prefix":"10.1002","volume":"164","author":[{"given":"Dalit","family":"Ashany","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith B.","family":"Elkon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Migliaccio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Rita","family":"Migliaccio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1992.tb05869.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V79.5.1185.1185"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1182\/blood.V74.4.1368.1368","article-title":"Murine thymocytes proliferate in direct response to interleukin\u20107","volume":"74","author":"Conlon P. 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