{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T09:10:45Z","timestamp":1698397845902},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3444,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The molecular basis of water\u2010permeability in salivary and other exocrine glands is not understood. We have examined two well\u2010studied salivary epithelial cell lines for evidence of a Hg\u2010inhibitable water\u2010permeability pathway. A5 and HSG cells are derived from rat and human submandibular glands, respectively. Only A5 cells are derived from rat and human submandibular glands, respectively. Only A5 cells demonstrated such a pathway. The rate of A5 cell osmotic shrinkage was inhibited about fivefold in the presence of 300 m\u0308M HgCl<jats:sub>2<\/jats:sub>. To determine if this activity was associated with the expression of the prototypical water channel (aquaporin, AQP) AQP1, we used three separate experimental approaches; Northern analysis and reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) analysis of isolated mRNA, and Western analysis of cell membranes. All three methods yielded positive results with A5 cells and negative results with HSG cells. The \u223c800 bp product of RT\u2010PCR was analyzed further by sequencing and restriction enzyme digestion. The results were consistent with the previously reported coding region sequence for rat kidney AQP1. The aggregate data demonstrate that marked differences in water\u2010permeability and water channel expression exist in these two salivary epithelial cell lines. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041640320","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T02:06:44Z","timestamp":1109383604000},"page":"613-619","source":"Crossref","is-referenced-by-count":6,"title":["Evidence for the presence of a Hg\u2010inhibitable water\u2010permeability pathway and aquaporin 1 in A5 salivary epithelial cells"],"prefix":"10.1002","volume":"164","author":[{"given":"Krzysztof W.","family":"Lazowski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Delporte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce J.","family":"Baum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1993.265.4.F463"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)92061-H"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_5_1","first-page":"1","volume-title":"Handbook of Physiology: The Gastrointestinal System III","author":"Cook D. 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