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B6.SJL and BALB\/c marrow cells were serially cultured with thrombopoietin (TPO), FLT\u20103 ligand (FLT\u20103L), and steel factor through cell cycle. Progenitors (high\u2010proliferative potential colony\u2010forming cells (HPP\u2010CFC) and colony\u2010forming unit culture (CFU\u2010c)) and ESC capacity was determined. The cell cycle status of purified lineage<jats:sup>negative<\/jats:sup>rhodamine<jats:sup>low<\/jats:sup>Hoechst<jats:sup>low<\/jats:sup> stem cells was determined under the same conditions using tritiated thymidine incorporation and cell counts. We found an inverse relationship between progenitors and ESC, which occurred during the first cell cycle transit and was reversible. We have termed these progenitor\/stem cell inversions and found that these inversions were consistently seen at 28\u201332 h of culture, representing early S\u2010phase. We observed 13 major reversible increases in progenitor numbers from one time\u2010point to another during the first cell cycle transit; this was coupled with 11 major ESC decreases and in 2 instances ESC were at baseline. These studies indicate that primitive marrow cells reversibly shift from ESC to progenitors without differentiation occurring. They exist as a fluctuating continuum. J. Cell. Physiol. 199: 20\u201331, 2004\u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.10436","type":"journal-article","created":{"date-parts":[[2004,2,18]],"date-time":"2004-02-18T10:00:30Z","timestamp":1077098430000},"page":"20-31","source":"Crossref","is-referenced-by-count":37,"title":["Intrinsic hematopoietic stem cell\/progenitor plasticity: Inversions"],"prefix":"10.1002","volume":"199","author":[{"given":"Gerald A.","family":"Colvin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Fran\u00e7ois","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian E.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane E.","family":"Carlson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark S.","family":"Dooner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehrdad","family":"Abedi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Cerny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Quesenberry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,10,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.10.3399"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-06-1780"},{"key":"e_1_2_5_4_1","first-page":"1","volume-title":"Statistical Methods in Medical Research","author":"Armitage P","year":"2001"},{"key":"e_1_2_5_5_1","first-page":"701","article-title":"Analysis and separation of murine bone marrow stem cells by H33342 fluorescence\u2010activated cell sorting","volume":"11","author":"Baines P","year":"1983","journal-title":"Exp Hematol"},{"key":"e_1_2_5_6_1","volume-title":"Clinical Flow Cytometry: Principals and Application","author":"Bauer KD","year":"1993"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1182\/blood.V26.3.296.296","article-title":"The effect of differing demands for blood cell production on DNA synthesis by hemopoietic colony\u2010forming cells of mice","volume":"26","author":"Becker AJ","year":"1965","journal-title":"Blood"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(98)00037-X"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(01)00734-2"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-76912-2_7"},{"key":"e_1_2_5_11_1","first-page":"265","article-title":"High proliferative potential colony\u2010forming cells","volume":"75","author":"Bertoncello I","year":"1997","journal-title":"Methods Mol Biol"},{"key":"e_1_2_5_12_1","first-page":"999","article-title":"Multiparameter analysis of transplantable hemopoietic stem cells: 0I. 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