{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:13:15Z","timestamp":1791400395330,"version":"4.3.3"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,1,21]],"date-time":"2004-01-21T00:00:00Z","timestamp":1074643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Some of the pathological manifestations of cystic fibrosis are in accordance with an impaired expression and\/or activity of PPAR\u03b3. We hypothesized that PPAR\u03b3 expression is altered in tissues lacking the normal cystic fibrosis transmembrane regulator protein (CFTR). PPAR\u03b3 mRNA levels were measured in colonic mucosa, ileal mucosa, adipose tissue, lung, and liver from wild\u2010type and cftr<jats:sup>\u2212\/\u2212<\/jats:sup> mice by quantitative RT\u2010PCR. PPAR\u03b3 expression was decreased twofold in CFTR\u2010regulated tissues (colon, ileum, and lung) from cftr<jats:sup>\u2212\/\u2212<\/jats:sup> mice compared to wild\u2010type littermates. In contrast, no differences were found in fat and liver. Immunohistochemical analysis of PPAR\u03b3 in ileum and colon revealed a predominantly nuclear localization in wild\u2010type mucosal epithelial cells while tissues from cftr<jats:sup>\u2212\/\u2212<\/jats:sup> mice showed a more diffuse, lower intensity labeling. A significant decrease in PPAR\u03b3 expression was confirmed in nuclear extracts of colon mucosa by Western blot analysis. In addition, binding of the PPAR\u03b3\/RXR heterodimer to an oligonucletotide containing a peroxisome proliferator responsive element (PPRE) was also decreased in colonic mucosa extracts from cftr<jats:sup>\u2212\/\u2212<\/jats:sup> mice. Treatment of cftr<jats:sup>\u2212\/\u2212<\/jats:sup> mice with the PPAR\u03b3 ligand rosiglitazone restored both the nuclear localization and binding to DNA, but did not increase RNA levels. We conclude that PPAR\u03b3 expression in cftr<jats:sup>\u2212\/\u2212<\/jats:sup> mice is downregulated at the RNA and protein levels and its function diminished. These changes may be related to the loss of function of CFTR and may be relevant to the pathogenesis of metabolic abnormalities associated with cystic fibrosis in humans. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20020","type":"journal-article","created":{"date-parts":[[2004,5,27]],"date-time":"2004-05-27T20:03:14Z","timestamp":1085688194000},"page":"235-244","source":"Crossref","is-referenced-by-count":55,"title":["Decreased expression of peroxisome proliferator activated receptor \u03b3 in CFTR<sup>\u2212\/\u2212<\/sup> mice"],"prefix":"10.1002","volume":"200","author":[{"given":"Mario","family":"Ollero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer","family":"Junaidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munir 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