{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:43:41Z","timestamp":1790729021107,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,5,19]],"date-time":"2004-05-19T00:00:00Z","timestamp":1084924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In a search for undiscovered mechanisms of resistance to adriamycin, we screened a genomic library derived from <jats:italic>Saccharomyces cerevisiae<\/jats:italic> for genes related to adriamycin resistance. To our surprise, we found that overexpression of <jats:italic>BSD2<\/jats:italic> rendered yeast cells resistant to adriamycin. Downregulation of the metal transporters Smf1 and Smf2 is the only activity of Bsd2 reported to date, and Bsd2 deficiency increases intracellular levels of Smf1 and Smf2. <jats:italic>SMF2<\/jats:italic>\u2010disrupted cells exhibited significantly greater resistance to adriamycin, whereas the resistance of <jats:italic>SMF1<\/jats:italic>\u2010disrupted cells was only slightly improved. The sensitivity of the <jats:italic>SMF1<\/jats:italic>\u2010 and <jats:italic>SMF2<\/jats:italic>\u2010disrupted yeast cell line overexpressing <jats:italic>BSD2<\/jats:italic> was almost the same as that of the <jats:italic>BSD2<\/jats:italic>\u2010overexpressing parental yeast cell. Thus the overexpression of <jats:italic>BSD2<\/jats:italic> and the disruption of <jats:italic>SMF1<\/jats:italic> and <jats:italic>SMF2<\/jats:italic> might be involved in the same mechanism that confers resistance to adriamycin. Although both <jats:italic>SMF1<\/jats:italic>\u2010 and <jats:italic>SMF2<\/jats:italic>\u2010disrupted cells were very sensitive to EGTA, overexpression of <jats:italic>BSD2<\/jats:italic> had little or no effect on sensitivity to EGTA. However, a partial decrease in the intracellular level of FLAG\u2010Smf2 was observed by overexpression of <jats:italic>BSD2<\/jats:italic>. Thus, the resistance to adriamycin acquired by overexpression of <jats:italic>BSD2<\/jats:italic> might be partially explained by down\u2010regulation of Smf2, but in addition to Smf2, other as of yet unidentified targets of Bsd2 must also be responsible for the resistance. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20082","type":"journal-article","created":{"date-parts":[[2004,5,19]],"date-time":"2004-05-19T17:01:48Z","timestamp":1084986108000},"page":"100-104","source":"Crossref","is-referenced-by-count":13,"title":["A novel role for Bsd2 in the resistance of yeast to 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