{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:17:01Z","timestamp":1762100221698},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,6,22]],"date-time":"2004-06-22T00:00:00Z","timestamp":1087862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Wild\u2010type mouse brain endothelial (bEND) cells transformed with the polyoma virus middle\u2010T proliferate rapidly in culture and form hemangiomas in mice. These cells express high levels of platelet\/endothelial cell adhesion molecule\u20101 (PECAM\u20101), a molecule shown to be important during hemangioma formation. In this study, we have examined the ability of polyoma virus middle\u2010T\u2010transformed mouse bEND cells prepared from PECAM\u20101\u2212\/\u2212 mice to proliferate in culture and form hemangiomas in mice. We show that these cells express a number of endothelial cell markers and share a similar morphology with PECAM\u20101+\/+ bEND cells. PECAM\u20101\u2212\/\u2212 bEND cells exhibit a limited ability to form tubes in Matrigel and rapidly form hemangioma when injected into nude mice, very similar to PECAM\u20101+\/+ bEND cells. These cells, however, have increased proliferation, slower migration, altered endothelial cell adhesion molecule expression, and are less adherent when compared to PECAM\u20101+\/+ bEND cells. Therefore, lack of PECAM\u20101 expression impacts polyoma middle\u2010T\u2010transformed endothelial cell proliferative, adhesive, and migratory properties without impacting their ability to rapidly form hemangiomas in mice or poorly organize to capillary\u2010like structures in Matrigel. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20114","type":"journal-article","created":{"date-parts":[[2004,6,22]],"date-time":"2004-06-22T20:00:49Z","timestamp":1087934449000},"page":"230-239","source":"Crossref","is-referenced-by-count":25,"title":["Polyoma virus middle\u2010T\u2010transformed PECAM\u20101 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice"],"prefix":"10.1002","volume":"202","author":[{"given":"Terri 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